Jing Siqun, Zhang Xiaoming, Yan Liang-Jun
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu 214122, China ; College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu 214122, China.
Oxid Med Cell Longev. 2015;2015:983484. doi: 10.1155/2015/983484. Epub 2015 Jan 1.
The objective of the present study was to evaluate the antioxidant activity, antitumor effect, and antiaging property of proanthocyanidins from Kunlun Chrysanthemum flowers (PKCF) grown in Xinjiang. In vitro antioxidant experiments results showed that the total antioxidant activity and the scavenging capacity of hydroxyl radicals ((•)OH) and 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)) radicals increased in a concentration-dependent manner and were stronger than those of vitamin C. To investigate the antioxidant activity of PKCF in vivo, we used serum, liver, and kidney from mouse for the measurement of superoxide dismutase (SOD), malondialdehyde (MDA), and total antioxidant capacity (T-AOC). Results indicated that PKCF had antioxidative effect in vivo which significantly improved the activity of SOD and T-AOC and decreased MDA content. To investigate the antitumor activity of PKCF, we used H22 cells, HeLa cells, and Eca-109 cells with Vero cells as control. Inhibition ratio and IC50 values were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; PKCF showed great inhibitory activity on H22 cells and HeLa cells. We also used fruit flies as a model for analyzing the anti-aging property of PKCF. Results showed that PKCF has antiaging effect on Drosophila. Results of the present study demonstrated that PKCF could be a promising agent that may find applications in health care, medicine, and cosmetics.
本研究的目的是评估新疆种植的昆仑菊花原花青素（PKCF）的抗氧化活性、抗肿瘤作用和抗衰老特性。体外抗氧化实验结果表明，总抗氧化活性以及羟基自由基（•OH）和1,1-二苯基-2-苦基肼自由基（DPPH•）的清除能力呈浓度依赖性增加，且强于维生素C。为研究PKCF在体内的抗氧化活性，我们使用小鼠的血清、肝脏和肾脏来测量超氧化物歧化酶（SOD）、丙二醛（MDA）和总抗氧化能力（T-AOC）。结果表明，PKCF在体内具有抗氧化作用，可显著提高SOD和T-AOC的活性，并降低MDA含量。为研究PKCF的抗肿瘤活性，我们使用H22细胞、HeLa细胞和Eca-109细胞，并以Vero细胞作为对照。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法测定抑制率和IC50值；PKCF对H22细胞和HeLa细胞表现出很强的抑制活性。我们还以果蝇为模型分析PKCF的抗衰老特性。结果表明，PKCF对果蝇具有抗衰老作用。本研究结果表明，PKCF可能是一种有前景的物质，可应用于保健品、医药和化妆品领域。
