Faculty of Training Administration, Department of Medical Education, The Fourth Military Medical University, Xi'an, People's Republic of China.
Shock. 2012 Jul;38(1):63-7. doi: 10.1097/SHK.0b013e3182596ec4.
Multiple organ failure is a common outcome of hemorrhagic shock followed by resuscitation, and the kidney is one of the prime target organs involved. The main objective of the study was to evaluate whether crocetin, a natural product from Gardenia jasminoides Ellis, has beneficial effects on renal dysfunction caused by hemorrhagic shock and resuscitation in rats. Anesthetized rats were bled to reduce mean arterial blood pressure to 35 (SD, 5) mmHg for 60 min and then were resuscitated with their withdrawn shed blood and normal saline. Crocetin was administered via the duodenum at a dose of 50 mg/kg 40 min after hemorrhage. The increase in creatinine and blood urea nitrogen was significantly reduced at 2 h after hemorrhage and resuscitation in crocetin-treated rats. The increases in renal nitric oxide, tumor necrosis factor α, and interleukin 6 were also attenuated by crocetin. Hemorrhagic shock resulted in a significant elevation in malondialdehyde production and was accompanied by a reduction in total superoxide dismutase activity, activation of nuclear factor κB, and overexpression of inducible nitric oxide synthase. These changes were significantly attenuated by crocetin at 2 h after resuscitation. These results suggested that crocetin blocks inflammatory cascades by inhibiting production of reactive oxygen species and restoring superoxide dismutase activity to ameliorate renal dysfunction caused by hemorrhage shock and resuscitation.
多器官衰竭是出血性休克后继发复苏的常见结果，肾脏是主要受累的靶器官之一。本研究的主要目的是评估藏红花酸（来自栀子的天然产物）是否对失血性休克和复苏后大鼠肾功能障碍有有益作用。麻醉大鼠放血，使平均动脉血压降低至 35（标准差，5）mmHg 持续 60 分钟，然后用抽出的失血和生理盐水进行复苏。出血后 40 分钟，通过十二指肠给予藏红花酸 50mg/kg。与对照组相比，在出血和复苏后 2 小时，藏红花酸治疗组的肌酐和血尿素氮升高明显减少。藏红花酸还可减轻肾组织中一氧化氮、肿瘤坏死因子-α和白细胞介素 6 的增加。出血性休克导致丙二醛产生显著增加，同时总超氧化物歧化酶活性降低，核因子κB 激活，诱导型一氧化氮合酶过度表达。这些变化在复苏后 2 小时可被藏红花酸显著减轻。这些结果表明，藏红花酸通过抑制活性氧的产生和恢复超氧化物歧化酶活性来阻断炎症级联反应，从而改善失血性休克和复苏引起的肾功能障碍。
