Michael Constantinos P, Derpapas Michael, Aravidou Eftychia, Sofopoulos Michael, Michael Panayiotis, Polydorou Andreas, Vezakis Antonios, Fragulidis Georgios P
Medicine, National and Kapodistrian University of Athens School of Medicine, Athens, GRC.
Breast Surgery, REA Maternity Hospital, Athens, GRC.
Cureus. 2020 Feb 13;12(2):e6979. doi: 10.7759/cureus.6979.
Renal impairment is associated with high mortality rates in severely ill patients. The need to prevent and treat renal damage underlines the importance of understanding the pathophysiological mechanisms that characterize it. This could also enable early diagnosis and the design of alternative therapeutic approaches. The aim of this study is to investigate the effect of crocetin, a known antioxidant, on the prevention of renal damage due to ischemia-reperfusion injury and the investigation of the mechanisms involved. The present study was performed on C57BL/6 mice aged 10-12 weeks. The animals had access to water and food ad libitum. The experiment, as described in materials and methods, was completed at 24 h, in which case the kidneys were removed for further study, both at tissue morphology (with immunohistochemistry) and changes in the level of miRs' expression by qRT-PCR. Accordingly, using the automatic precision analyzer, the serum levels of the basic parameters currently used clinically for the monitoring of renal function were determined. The administration of crocetin, despite the short presence of the substance in the body, affects all the biochemical parameters analyzed (urea, creatinine, uric acid, and ions of Na, K, Cl, P, Mg and Ca), causing significant decrease of their measured values. Crocetin also resulted in a significant limitation of the inflammation elements and the degree of epithelial damage. Furthermore, the administration of crocetin appears to restore levels of expression of miR21, miR127 and miR132.
肾功能损害与重症患者的高死亡率相关。预防和治疗肾损伤的必要性凸显了了解其病理生理机制的重要性。这也有助于早期诊断和设计替代治疗方法。本研究的目的是研究已知的抗氧化剂藏红花素对预防缺血再灌注损伤所致肾损伤的作用，并探究其相关机制。本研究以10 - 12周龄的C57BL/6小鼠为实验对象。动物可自由获取水和食物。如材料与方法中所述，实验在24小时时完成，此时取出肾脏进行进一步研究，包括组织形态学（采用免疫组织化学）以及通过qRT-PCR检测miR表达水平的变化。相应地，使用自动精密分析仪测定目前临床上用于监测肾功能的基本参数的血清水平。尽管藏红花素在体内存在时间较短，但它对所有分析的生化参数（尿素、肌酐、尿酸以及钠、钾、氯、磷、镁和钙的离子）都有影响，导致其测量值显著降低。藏红花素还显著限制了炎症因子和上皮损伤程度。此外，给予藏红花素似乎能恢复miR21、miR127和miR132的表达水平。
