Di Paolo N, Vanni L, Sacchi G
Nephrology and Dialysis Department, Regional Hospital of Siena, Italy.
Clin Nephrol. 1990 Oct;34(4):179-84.
With the purpose of studying peritoneal physiology, original biopsy methods were devised and human and rabbit peritoneal mesothelial cells cultured and characterized. It was then decided to verify whether these cells could be implanted autologously during peritoneal dialysis in cases of acute loss of mesothelial surface. Staphylococcal peritonitis was induced in 12 rabbits and after 4 days of antibiotics, 6 of them were autoimplanted with cultured mesothelial cells, previously marked in 3 cases with thymidine (H3TdR). Implanted rabbits sacrificed after 3 and 6 days showed taking of the new mesothelial cells both by direct morphological observation and by autoradiography. Four uremic CAPD patients recovering from severe peritonitis were implanted with 300 million of their own peritoneal mesothelial cells, previously cultured and frozen. Morphological signs of taking were evident by laparoscopy and from peritoneal biopsies performed 3 and 6 days after implant. The fact that such implants are possible may have interesting applications in medicine and surgery.
为了研究腹膜生理学，设计了原始活检方法，并对人和兔的腹膜间皮细胞进行培养和鉴定。随后决定验证在间皮表面急性缺失的情况下，这些细胞能否在腹膜透析期间进行自体植入。对12只兔子诱发葡萄球菌性腹膜炎，在使用抗生素4天后，其中6只自体植入培养的间皮细胞，3例之前用胸苷（H3TdR）进行了标记。植入后3天和6天处死的兔子，通过直接形态学观察和放射自显影显示新的间皮细胞已被接纳。4例从严重腹膜炎中康复的尿毒症持续性不卧床腹膜透析（CAPD）患者，植入了3亿个他们自己先前培养并冷冻保存的腹膜间皮细胞。植入后3天和6天通过腹腔镜检查和腹膜活检可见明显的接纳形态学迹象。这种植入的可行性这一事实可能在医学和外科手术中有有趣的应用。
