Di Paolo N, Sacchi G, Vanni L, Corazzi S, Terrana B, Rossi P, Gaggiotti E, Buoncristiani U
Nephrology and Dialysis Department, Regional Hospital of Siena, Italy.
Nephron. 1991;57(3):323-31. doi: 10.1159/000186283.
Human and rabbit peritoneal mesothelial cells have been successfully cultured and autoimplanted. An original biopsy technique was used to take samples of peritoneal mesothelial cells and after culture they were characterized from the structural and caryological points of view. Staphylococcal peritonitis was induced in 12 rabbits with indwelling peritoneal catheters and after 4 days of antibiotics 6 of them were autoimplanted with cultured autologous mesothelial cells previously marked in 3 cases with thymidine (H3TdR). Direct morphological observation and autoradiography were used to compare the mesothelium of control rabbits and implanted rabbits sacrificed on days 3 and 6 and showed that the cell implants had taken. Four uremic CAPD patients recovering from severe peritonitis were implanted with about 300 million autologous peritoneal mesothelial cells, previously cultured and frozen. Morphological signs of taking were evident from peritoneal laparoscopy biopsies performed 3 and 6 days after implant. The aim of the study was merely to demonstrate that such implants are possible; however, the techniques may have interesting applications not only in peritoneal dialysis, but also in the vaster fields of medicine and surgery.
人及兔腹膜间皮细胞已成功培养并自体移植。采用一种独创的活检技术获取腹膜间皮细胞样本，培养后从结构和细胞学角度对其进行表征。给12只留置腹膜导管的兔诱发葡萄球菌性腹膜炎，在使用抗生素4天后，其中6只自体移植了先前在3例中用胸苷（H3TdR）标记的培养自体间皮细胞。使用直接形态学观察和放射自显影术比较在第3天和第6天处死的对照兔和移植兔的间皮，结果显示细胞植入成功。4例从严重腹膜炎中康复的尿毒症持续性非卧床腹膜透析（CAPD）患者移植了约3亿个先前培养并冷冻的自体腹膜间皮细胞。在植入后第3天和第6天进行的腹膜腹腔镜活检显示有明显的植入迹象。该研究的目的仅仅是证明这种植入是可行的；然而，这些技术不仅可能在腹膜透析中有有趣的应用，而且在更广泛的医学和外科领域也可能有应用。
