Department of Pharmacognosy and Pharmaceutical Botany , Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.
Nat Prod Res. 2013 Apr;27(7):684-6. doi: 10.1080/14786419.2012.686912. Epub 2012 May 14.
This study evaluated microwave-assisted extraction (MAE) for four pentacyclic triterpenes as well as developed a method for preparing a pentacyclic triterpenene rich extract from Centella asiatica. MAE was capable of increasing the yield of the pentacyclic triterpenes up to twice that produced by the heat reflux method, and it was also much less time consuming. The optimal conditions of MAE employed were extraction with absolute ethanol as solvent, an irradiation power of 600 W, at 75°C, four irradiation cycles and four extraction times. Here, we provide a simple method for the preparation of the pentacyclic triterpene rich C. asiatica extracts, which contained not less than 65% w/w total pentacyclic triterpenes. The method involved a macroporous resin (Diaion® HP-20) column eluted with ethanol and a decolourisation step with activated charcoal.
本研究评估了微波辅助提取（MAE）四种五环三萜的效果，并开发了一种从积雪草中制备五环三萜丰富提取物的方法。MAE 能够将五环三萜的产率提高至热回流法的两倍，且所需时间也大大缩短。MAE 的最佳条件为以无水乙醇为溶剂，辐射功率 600W，75°C 下进行四次辐射循环和四次提取。在此，我们提供了一种简单的方法来制备五环三萜丰富的积雪草提取物，其中含有不少于 65%（w/w）的总五环三萜。该方法涉及用乙醇洗脱的大孔树脂（Diaion® HP-20）柱和用活性炭进行的脱色步骤。
