异生物素对大鼠胆汁流量和胆汁成分的影响——方法学探讨

Influence of xenobiotics on bile flow and bile composition in rats--methodological approach.

作者信息

Fleck C, Barth A

机构信息

Friedrich Schiller University, Institute of Pharmacology and Toxicology, Jena, GDR.

出版信息

Exp Pathol. 1990;39(3-4):175-85. doi: 10.1016/s0232-1513(11)80181-7.

DOI:10.1016/s0232-1513(11)80181-7

PMID:2257922

Abstract

Optimal procedures for the investigation of bile flow and excretion of bile constituents are described, and data are given regarding sex and age dependency, use of narcotic drugs and replacement of water loss in Wistar rats. A combination of ketamine and xylazine can be recommended for anaesthesia. In long time studies saline infusion keeps bile and urine production constant over a period of 6 h. Bile flow and biliary excretion of bile acids and electrolytes are immature at birth and reach a maximum between the 20th and 60th day of life. The biliary excretion of cholesterol decreases with age. The concentrations of bile constituents such as lipids, glutathione, protein, uric acid, urea, osmotically active substances, and steroid hormones are given for adult rats. Bilateral nephrectomy decreases bile flow in mature rats only.

摘要

本文描述了研究胆汁流动和胆汁成分排泄的最佳程序，并给出了有关Wistar大鼠性别和年龄依赖性、麻醉药物使用以及水分流失补充的数据。氯胺酮和赛拉嗪联合使用可推荐用于麻醉。在长期研究中，生理盐水输注可使胆汁和尿液生成在6小时内保持恒定。出生时胆汁流动以及胆汁酸和电解质的胆汁排泄不成熟，在出生后第20至60天达到最大值。胆固醇的胆汁排泄随年龄增长而减少。给出了成年大鼠胆汁成分如脂质、谷胱甘肽、蛋白质、尿酸、尿素、渗透活性物质和类固醇激素的浓度。双侧肾切除仅会降低成熟大鼠的胆汁流动。