Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul, South Korea.
Cytokine. 2012 Aug;59(2):237-44. doi: 10.1016/j.cyto.2012.04.021. Epub 2012 May 12.
Parasitic helminths are well-known to have the ability to modulate host immune responses. In this study, we investigated the fundamental immunoregulatory mechanism of the liver fluke Clonorchis sinensis (C. sinensis) using a murine macrophage RAW 264.7 (RAW) cell line and mouse bone marrow derived macrophages (BMDMs). We found that C. sinensis crude antigen (CA) is able to differentiate macrophage RAW cells into dendritic-like cells that can be detected by morphological observations. In addition, CA induces prominent secretion of anti-inflammatory cytokines such as IL-10 and TGF-β; however, we did not observe changes in cell surface markers that are involved in antigen recognition, antigen presentation, and T cell activation. Additionally, CA treatment induced ERK and JNK phosphorylation, and unexpectedly, elevated levels of IL-10 and TGF-β were inhibited by the addition of an ERK-specific inhibitor. Taken together, these data demonstrate that CA from C. sinensis may modulate host immune responses by upregulating anti-inflammatory cytokines via the regulation of ERK.
寄生虫蠕虫被广泛认为具有调节宿主免疫反应的能力。在这项研究中，我们使用鼠巨噬细胞 RAW 264.7（RAW）细胞系和鼠骨髓来源的巨噬细胞（BMDM）来研究肝吸虫（Clonorchis sinensis，C. sinensis）的基本免疫调节机制。我们发现，肝吸虫粗抗原（CA）能够将巨噬细胞 RAW 细胞分化为树突状样细胞，这可以通过形态学观察来检测。此外，CA 诱导明显的抗炎细胞因子如 IL-10 和 TGF-β的分泌；然而，我们没有观察到参与抗原识别、抗原呈递和 T 细胞激活的细胞表面标志物的变化。此外，CA 处理诱导 ERK 和 JNK 的磷酸化，出人意料的是，添加 ERK 特异性抑制剂可抑制 IL-10 和 TGF-β 的水平升高。综上所述，这些数据表明，来自肝吸虫的 CA 可能通过调节 ERK 来上调抗炎细胞因子来调节宿主免疫反应。
