Department of Biology, Institute of Molecular Systems Biology, Zurich, Switzerland.
J Proteomics. 2012 Jun 27;75(12):3495-513. doi: 10.1016/j.jprot.2012.04.048. Epub 2012 May 8.
Due to the enormous complexity of proteomes which constitute the entirety of protein species expressed by a certain cell or tissue, proteome-wide studies performed in discovery mode are still limited in their ability to reproducibly identify and quantify all proteins present in complex biological samples. Therefore, the targeted analysis of informative subsets of the proteome has been beneficial to generate reproducible data sets across multiple samples. Here we review the repertoire of antibody- and mass spectrometry (MS) -based analytical tools which is currently available for the directed analysis of predefined sets of proteins. The topics of emphasis for this review are Selected Reaction Monitoring (SRM) mass spectrometry, emerging tools to control error rates in targeted proteomic experiments, and some representative examples of applications. The ability to cost- and time-efficiently generate specific and quantitative assays for large numbers of proteins and posttranslational modifications has the potential to greatly expand the range of targeted proteomic coverage in biological studies. This article is part of a Special Section entitled: Understanding genome regulation and genetic diversity by mass spectrometry.
由于蛋白质组的巨大复杂性，它构成了特定细胞或组织表达的所有蛋白质种类，因此在发现模式下进行的蛋白质组范围的研究仍然难以重复地识别和定量复杂生物样本中存在的所有蛋白质。因此，针对蛋白质组的信息子集进行有针对性的分析对于在多个样本中生成可重复的数据组是有益的。在这里，我们回顾了目前可用于定向分析预定蛋白质组的抗体和质谱 (MS) 分析工具的范围。本综述的重点是选择反应监测 (SRM) 质谱、控制靶向蛋白质组学实验中错误率的新兴工具以及一些应用的代表性示例。能够高效且经济地为大量蛋白质和翻译后修饰物生成特定和定量的测定法，有可能极大地扩展生物研究中靶向蛋白质组学覆盖范围。本文是一个专题的一部分，该专题题为：通过质谱理解基因组调控和遗传多样性。
