Lorentz Holly, Heynen Miriam, Trieu Diana, Hagedorn Sarah J, Jones Lyndon
Centre for Contact Lens Research, School of Optometry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada.
Optom Vis Sci. 2012 Jun;89(6):856-67. doi: 10.1097/OPX.0b013e318255ddc8.
To analyze the influence of various tear film components on in vitro deposition of two lipids (cholesterol and phosphatidylcholine) on three contact lens materials.
Etafilcon A, balafilcon A, and senofilcon A were incubated in four different incubation solutions for 3 or 14 days: an artificial tear solution containing lipids and proteins, a protein tear solution containing proteins and the lipid of interest, a lipid tear solution containing lipids and no proteins, and a single lipid tear solution containing the lipid of interest only. Each incubation solution contained one of the two radiolabeled lipids: C-cholesterol (C) or C-phosphatidylcholine (PC). After soaking, lenses were removed from the incubation solution, the lipids were extracted and quantified using a beta counter, and masses of lipid were calculated using standard calibration curves.
This experiment examined several different parameters influencing lipid deposition on contact lenses, including lens material, length of incubation, and the composition of the incubation solution. Overall, lipid deposited differently on different lens materials (p < 0.0005), with the order of deposition most commonly being balafilcon > senofilcon > etafilcon. Incubation solution had a large impact on how much lipid was deposited (p < 0.00001), although cholesterol and phosphatidylcholine demonstrated different deposition patterns. Lipid deposition after 14 days of incubation was consistently greater than after 3 days (p < 0.02).
This in vitro study demonstrates that C and PC deposition are cumulative over time and that silicone hydrogel materials deposit more lipid than group IV conventional hydrogel materials. It also clearly demonstrates that deposition of C and PC is influenced by the composition of the incubation solution and that in vitro models must use more physiologically relevant incubation solutions that mimic the natural tear film if in vitro data is to be extrapolated to the in vivo situation.
分析各种泪膜成分对两种脂质（胆固醇和磷脂酰胆碱）在三种隐形眼镜材料上的体外沉积的影响。
将依他氟康A、百利氟康A和森氟康A在四种不同的孵育溶液中孵育3天或14天：一种含脂质和蛋白质的人工泪液溶液、一种含蛋白质和目标脂质的蛋白质泪液溶液、一种含脂质但不含蛋白质的脂质泪液溶液以及一种仅含目标脂质的单一脂质泪液溶液。每种孵育溶液含有两种放射性标记脂质中的一种：¹⁴C -胆固醇（¹⁴C）或³²P -磷脂酰胆碱（³²PC）。浸泡后，将镜片从孵育溶液中取出，提取脂质并使用β计数器进行定量，然后使用标准校准曲线计算脂质质量。
本实验研究了影响脂质在隐形眼镜上沉积的几个不同参数，包括镜片材料、孵育时间和孵育溶液的组成。总体而言，脂质在不同镜片材料上的沉积方式不同（p < 0.0005），最常见的沉积顺序为百利氟康>森氟康>依他氟康。孵育溶液对脂质沉积量有很大影响（p < 0.00001），尽管胆固醇和磷脂酰胆碱表现出不同的沉积模式。孵育14天后的脂质沉积量始终大于3天后的沉积量（p < 0.02）。
这项体外研究表明，¹⁴C和³²PC的沉积随时间累积，并且硅水凝胶材料比IV组传统水凝胶材料沉积更多的脂质。它还清楚地表明，¹⁴C和³²PC的沉积受孵育溶液组成的影响，并且如果要将体外数据外推到体内情况，体外模型必须使用更模拟天然泪膜的生理相关孵育溶液。
Zotero 插件