Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, 61801, USA.
Poult Sci. 2012 Jun;91(6):1350-5. doi: 10.3382/ps.2011-01861.
The objective of this study was to determine amino acid digestibility of 4 feedstuffs [soybean meal (SBM), canola meal, fish meal, and meat and bone meal (MBM)] using the precision-fed cecectomized rooster assay (PFR), the standardized ileal assay (SIAAD), and a newly developed precision-fed ileal broiler assay (PFC). For the PFR, cecectomized roosters were precision-fed approximately 30 g of feed sample, and excreta were collected 48 h postfeeding. For the SIAAD, 16-d-old broilers were fed a semipurified diet containing the feed samples as the only source of protein from 17 to 21 d, with ileal digesta collected at 21 d. For the PFC, 22-d-old broilers were precision-fed 10 g of feed sample mixed with chromic oxide, and ileal digesta were collected at 4 h postfeeding. Digestibility coefficients were standardized using a nitrogen-free diet for the SIAAD and PFC and using fasted roosters for the PFR. There were generally no consistent differences in standardized amino acid digestibility values among assays, and values were in general agreement among assays, particularly for SBM and MBM. Differences did occur among methods for amino acid digestibility in fish meal; however, these differences were not consistent among methods or amino acids. The results of the study indicated that all 3 bioassays are acceptable for determining the amino acid digestibility of SBM, canola meal, MBM, and fish meal for poultry.
本研究旨在使用精确饲喂盲肠去盲肠公鸡测定法（PFR）、标准回肠法（SIAAD）和新开发的精确饲喂肉鸡回肠法（PFC）来测定 4 种饲料[豆粕（SBM）、菜粕、鱼粉和肉骨粉（MBM）]的氨基酸消化率。对于 PFR，盲肠去盲肠公鸡被精确饲喂约 30 g 饲料样品，在饲喂后 48 h 收集粪便。对于 SIAAD，16 日龄肉鸡饲喂含有饲料样品的半纯化日粮，作为 17 至 21 d 唯一蛋白质来源，在 21 d 时收集回肠消化物。对于 PFC，22 日龄肉鸡被精确饲喂 10 g 混合有氧化铬的饲料样品，在饲喂后 4 h 收集回肠消化物。SIAAD 和 PFC 采用无氮日粮对消化率进行标准化，PFR 采用空腹公鸡进行标准化。在不同测定方法中，标准化氨基酸消化率值通常没有一致的差异，并且各测定方法之间的结果基本一致，尤其是 SBM 和 MBM。鱼粉中氨基酸消化率在不同方法之间确实存在差异；然而，这些差异在方法或氨基酸之间并不一致。研究结果表明，所有 3 种生物测定法均可用于测定家禽 SBM、菜粕、MBM 和鱼粉的氨基酸消化率。
