Han Xiuwu, Zhang Bao, Yan Wei, Zhao Zhiwei, Gao Qiang, Zhang Yuhai
Kidney Transplant Centre, Department of Urology, Beijing Shijitan Hospital, Affiliate to Capital Medical University, Beijing 100038, PR China.
Exp Clin Transplant. 2012 Dec;10(6):573-8. doi: 10.6002/ect.2012.0005. Epub 2012 May 14.
To laparoscopically compare the effectiveness of 2 cooling devices for renal hypothermia and investigate the feasibility of laparoscopic orthotopic kidney transplant using a pig model.
Eight pigs were divided into 2 groups of 4 animals each. Laparoscopic nephrectomy and autotransplant were performed first on only the right kidney. One week later, these procedures were performed on the left kidney, while the first transplanted autograft was removed. After 1 more week, the left autograft was removed for observation. In 1 group, the silicon tube cage was used to induce hypothermia during laparoscopic orthotopic kidney transplant (silicon tube cage group), and in the other group, the plastic bag jacket was used to induce hypothermia during laparoscopic orthotopic kidney transplant (plastic bag jacket group).
Two pigs in the silicon tube cage group survived for 7 days after the second autotransplant with serum creatinine levels of 210 μmol/L and 1010 μmol/L. One pig in the plastic bag jacket group survived for 5 days. The mean surface temperature of the grafts was maintained at 9°C ± 3°C and 12°C ± 3°C in the silicon tube cage and plastic bag jacket groups (P = .166). Three of 6 plastic bag jacket devices were ruptured by stitches or instruments. The mean venous and arterial anastomotic times in the silicon tube cage group were significantly shorter than were those in the plastic bag jacket group.
Our study shows that the silicon tube cage may be a reliable renal cooling device for use in laparoscopic kidney transplant and indicates the feasibility of laparoscopic orthotopic kidney transplant in pigs.
通过腹腔镜比较两种肾低温冷却装置的有效性，并利用猪模型研究腹腔镜原位肾移植的可行性。
将8头猪分为两组，每组4只。首先仅对右侧肾脏进行腹腔镜肾切除术和自体移植。一周后，对左侧肾脏进行这些操作，同时切除首次移植的自体移植物。再过一周后，取出左侧自体移植物进行观察。一组在腹腔镜原位肾移植期间使用硅胶管笼诱导低温（硅胶管笼组），另一组在腹腔镜原位肾移植期间使用塑料袋套诱导低温（塑料袋套组）。
硅胶管笼组有2头猪在第二次自体移植后存活7天，血清肌酐水平分别为210 μmol/L和1010 μmol/L。塑料袋套组有1头猪存活5天。硅胶管笼组和塑料袋套组移植物的平均表面温度分别维持在9°C ± 3°C和12°C ± 3°C（P = 0.166）。塑料袋套组6个装置中有3个被缝线或器械弄破。硅胶管笼组的平均静脉和动脉吻合时间明显短于塑料袋套组。
我们的研究表明，硅胶管笼可能是一种用于腹腔镜肾移植的可靠肾冷却装置，并表明猪腹腔镜原位肾移植的可行性。
