Braun Hans-Georg, Cardoso André Zamith
Leibniz Institut of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany.
Colloids Surf B Biointerfaces. 2012 Sep 1;97:43-50. doi: 10.1016/j.colsurfb.2012.03.028. Epub 2012 Apr 27.
Liquid marbles made from Lycopodium clavatum spores are used to encapsulate aqueous solutions of 9-fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF). Acidification of the Fmoc-FF solution at the liquid/air interface of the liquid marble triggers the self-assembly of ribbon-like peptide fibrils into an ultrathin peptide membrane (50-500 nm). The membrane incorporates the lycopodium microparticles and as a result stabilizes the liquid marble against collapse, that could otherwise occur through particle disintegration at the floating interphase. Ultrathin nanostructured peptide membrane formation at the liquid/air interface is also observed within artificial microstructured floating objects. Thus, peptide membranes formed were inspected by SEM and TEM. Electron diffraction data reveal information about the molecular organization inside the oligopeptide membranes.
由石松孢子制成的液滴用于封装9-芴甲氧羰基-二苯基丙氨酸（Fmoc-FF）的水溶液。在液滴的液/气界面处对Fmoc-FF溶液进行酸化，会触发带状肽原纤维自组装成超薄肽膜（50 - 500纳米）。该膜包含石松微粒，从而稳定液滴防止其坍塌，否则液滴可能会因漂浮界面处的颗粒分解而坍塌。在人工微结构漂浮物体内也观察到在液/气界面形成超薄纳米结构肽膜。因此，通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）对形成的肽膜进行了检查。电子衍射数据揭示了寡肽膜内部分子组织的信息。
