Celebier Mustafa, Ibáñez Clara, Simó Carolina, Cifuentes Alejandro
Laboratory of Foodomics, CIAL, CSIC, Madrid, Spain.
Methods Mol Biol. 2012;869:185-95. doi: 10.1007/978-1-61779-821-4_15.
The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.
通过对经膳食多酚处理和未处理的人HT29结肠癌细胞的代谢物进行分析，证明了毛细管电泳-质谱联用（CE-MS）在代谢组学中的潜力。在进行CE-MS分析之前，研究了四种不同的代谢物纯化策略。具体而言，描述了甲醇脱蛋白、超滤以及使用C18和聚合物基柱的两种固相萃取方法后获得的结果。这些通用方法可广泛应用于分析各种基质和领域中的代谢物，包括新的食品组学领域。
