聚丙烯酰胺凝胶电泳后量子点对人血清蛋白进行四甲基乙二胺增强的光致发光成像
TEMED-enhanced photoluminescent imaging of human serum proteins by quantum dots after PAGE.
作者信息
Na Na, Ouyang Jin
机构信息
Beijing Normal University, Beijing, People's Republic of China.
出版信息
Methods Mol Biol. 2012;869:511-20. doi: 10.1007/978-1-61779-821-4_45.
Polyacrylamide gel electrophoresis (PAGE) has been one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. As a simple, convenient, and ultrasensitive detection of proteins for PAGE, a novel enhanced photoluminescent (PL) imaging method was developed. Thioglycolic acid (TGA)-capped CdTe quantum dots (QDs) and the enhanced reagent of tetramethylethylenediamine (TEMED) are introduced, achieving the direct detection of various proteins in native 1-DE, 2-DE, and SDS gels. Here, we describe the general protocol of TEMED-enhanced PL imaging by QDs, including materials, practical procedures, as well as some notes.
聚丙烯酰胺凝胶电泳（PAGE）一直是用于复杂生物样品的最强大且应用最广泛的分离技术之一，其传统检测方法包括有机染料染色或银染。作为一种用于PAGE的简单、便捷且超灵敏的蛋白质检测方法，人们开发了一种新型增强光致发光（PL）成像方法。引入了巯基乙酸（TGA）包覆的碲化镉量子点（QDs）和四甲基乙二胺（TEMED）增强试剂，实现了对天然一维电泳、二维电泳和十二烷基硫酸钠（SDS）凝胶中各种蛋白质的直接检测。在此，我们描述了量子点增强的TEMED光致发光成像的一般方案，包括材料、实际操作步骤以及一些注意事项。
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