The Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Talanta. 2012 Sep 15;99:939-43. doi: 10.1016/j.talanta.2012.07.062. Epub 2012 Aug 1.
We report a conjugate of thioglycolic acid (TGA) capped CdTe quantum dot and Eu(3+) ion (TGA-CdTe QD-Eu(3+)) that can be used as an ultrasensitive luminescence turn-on sensor for nucleoside triphosphates (NTPs). The TGA-CdTe QD-Eu(3+) conjugate is a weakly luminescent species as a result of the strong quenching effect of Eu(3+) ion on the luminescence of TGA-CdTe QDs. The conjugate's luminescence can be readily restored by its reaction with adenosine triphosphate (ATP) and other NTPs, and thus gives an ultrasensitive detection of NTPs, with a detection limit of 2 nM. The sensing mechanism has also been explored, and the effective quenching of TGA-CdTe QDs emission by Eu(3+) ions has been attributed to photoinduced electron transfer (PET). ATP, as the representative of NTPs, can remove Eu(3+) from the surface of TGA-CdTe QDs, leading to restoration of the TGA-CdTe QDs luminescence.
我们报告了一种巯基乙酸（TGA）封端的碲化镉量子点和 Eu(3+)离子（TGA-CdTe QD-Eu(3+)）的缀合物，可作为核苷三磷酸（NTP）的超灵敏荧光开启传感器。TGA-CdTe QD-Eu(3+)缀合物的荧光很弱，这是由于 Eu(3+)离子对 TGA-CdTe QDs 荧光的强烈猝灭作用所致。缀合物的荧光可以通过与三磷酸腺苷（ATP）和其他 NTP 的反应轻易恢复，从而对 NTP 进行超灵敏检测，检测限低至 2 nM。还探索了传感机制，Eu(3+)离子对 TGA-CdTe QDs 发射的有效猝灭归因于光诱导电子转移（PET）。作为 NTP 的代表，ATP 可以从 TGA-CdTe QDs 表面去除 Eu(3+)，从而恢复 TGA-CdTe QDs 的荧光。
