Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810001, PR China.
Phytochem Anal. 2012 Nov-Dec;23(6):684-8. doi: 10.1002/pca.2373. Epub 2012 May 14.
The official rhubarb species have frequently been investigated for their hydroxyanthraquinone components and associated pharmacological properties. However, other unofficial rhubarb species were rarely studied until polyhydroxystilbenes (PHS), which commonly occur in the unofficial rhubarb, revealed a range of potential bioactivities. Hence, there has been increasing interest in the efficient preparation of high-purity PHS for pharmacological and clinical trials.
To develop a suitable method for large-scale preparative separation of PHS from the rhizome of Rheum likiangense Sam. by high-speed counter-current chromatography (HSCCC).
Two PHS compounds were isolated successfully within 440 min using a solvent system consisting of methanol:n-butanol:chloroform:water (2:0.5:3:3, v/v/v/v). Eighty-four milligrams of desoxyrhaponticin with 98.2% purity and 148 mg of rhaponticin with 95.3% purity were respectively yielded from 1.5 g of crude extract in the single, one-step operation.
An optimised HSCCC method has been established for large-scale preparative separation of PHS compounds from Rheum likiangense Sam.
官方大黄属植物因其羟基蒽醌成分和相关的药理特性而经常受到研究。然而，直到非官方大黄属植物中普遍存在的多羟基芪（PHS）揭示了一系列潜在的生物活性，其他非官方大黄属植物才很少被研究。因此，人们对高效制备用于药理和临床试验的高纯度 PHS 的兴趣日益浓厚。
通过高速逆流色谱（HSCCC）从丽江大黄根状茎中开发一种适合大规模制备分离 PHS 的方法。
使用甲醇：正丁醇：氯仿：水（2:0.5:3:3，v/v/v/v）的溶剂系统，成功地在 440 分钟内分离出两种 PHS 化合物。从 1.5 克粗提物中，在单次一步操作中分别得到了 84 毫克纯度为 98.2%的去氧虎杖苷和 148 毫克纯度为 95.3%的虎杖苷。
已经建立了一种优化的 HSCCC 方法，用于从丽江大黄根状茎中大规模制备分离 PHS 化合物。
