Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
J Anim Sci. 2012 Nov;90(11):3798-808. doi: 10.2527/jas.2011-4692. Epub 2012 May 14.
The objectives of this experiment were to evaluate procedures that may be used to predict the concentration of standardized ileal digestible (SID) Lys in distillers dried grains with solubles (DDGS) fed to pigs and to evaluate the accuracy of a published equation to predict SID Lys in DDGS. Twenty-one sources of DDGS were analyzed (as-fed basis) for CP (23.8% to 33.6%; CV = 8.3%), Lys (0.69% to 1.17%; CV = 12.4%), and furosine (0.02% to 0.22%; CV = 91.4%). The concentration of reactive Lys (%, as-fed basis) was calculated as analyzed Lys (%) - furosine (%) ÷ 0.32 × 0.40 and ranged from 0.47% to 1.15% (CV = 20.7%) in the 21 sources of DDGS. Twenty-one diets that each contained 60.0% of 1 source of DDGS as the sole source of CP and AA were formulated. An N-free diet was also formulated and was used to determine basal endogenous losses of CP and AA. Twenty-two barrows with an initial BW of 45.2 kg (SD = 3.1 kg) were fitted with a T-cannula in the distal ileum and allotted to a 22 × 10 Youden square design with the 22 diets and 10 periods. The SID of CP and AA were calculated for each source of DDGS. The SID of CP ranged from 69.8% to 79.6%, and the SID of Lys ranged from 45.3% to 74.1%. The concentration of SID Lys in the 21 samples of DDGS was highly related to the concentration of analyzed Lys (P < 0.001; r(2) = 0.849) and with the concentration of reactive Lys in the samples (P < 0.001; r(2) = 0.898). In contrast, the concentration of SID Lys in the 21 sources of DDGS was not related to the concentration of CP in the samples (P = 0.558; r(2) = 0.021). However, values for SID Lys were in good agreement with values predicted using a published prediction equation. In conclusion, analyzed Lys in DDGS, but not CP, may be used to predict the concentration of SID Lys in DDGS fed to pigs. However, analysis of furosine in addition to Lys and subsequent calculation of reactive Lys improve the prediction accuracy of digestible Lys concentration in DDGS.
本实验的目的是评估可能用于预测猪饲用酒糟（DDGS）中标准化回肠可消化赖氨酸（SID Lys）浓度的方法，并评估一种已发表的预测 DDGS 中 SID Lys 方程的准确性。对 21 种 DDGS 来源（原样）进行 CP（23.8%至 33.6%；变异系数=8.3%）、Lys（0.69%至 1.17%；变异系数=12.4%）和呋喃氨酸（0.02%至 0.22%；变异系数=91.4%）分析。原样基础上，反应性 Lys（%）的浓度计算为分析 Lys（%）-呋喃氨酸（%）÷0.32×0.40，21 种 DDGS 来源的反应性 Lys 浓度范围为 0.47%至 1.15%（变异系数=20.7%）。配制 21 种饲粮，每种饲粮均以 1 种 DDGS 来源作为 CP 和 AA 的唯一来源，配制无氮饲粮以确定 CP 和 AA 的基础内源损失。22 头初始体重为 45.2kg（SD=3.1kg）的阉公猪在回肠末端安装 T 型瘘管，采用 22×10 Youden 正方形设计，采用 22 种饲粮和 10 个周期进行试验。计算每个 DDGS 来源的 CP 和 AA 的 SID。CP 的 SID 范围为 69.8%至 79.6%，Lys 的 SID 范围为 45.3%至 74.1%。21 个 DDGS 样品中 SID Lys 的浓度与分析 Lys 的浓度高度相关（P＜0.001；r²=0.849），与样品中反应性 Lys 的浓度也高度相关（P＜0.001；r²=0.898）。相比之下，21 个 DDGS 来源的 SID Lys 浓度与样品中的 CP 浓度无关（P=0.558；r²=0.021）。然而，SID Lys 的值与使用已发表的预测方程预测的值非常吻合。总之，DDGS 中的分析 Lys（而非 CP）可用于预测猪饲用 DDGS 中的 SID Lys 浓度。然而，除 Lys 外，对呋喃氨酸进行分析并随后计算反应性 Lys，可提高 DDGS 中可消化 Lys 浓度的预测准确性。
