National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, 305-8518, Japan.
Amino Acids. 2012 Dec;43(6):2393-416. doi: 10.1007/s00726-012-1319-6. Epub 2012 May 16.
A comparative proteomic study was performed to unravel the protein networks involved in cadmium stress response in soybean. Ten-day-old seedlings of contrasting cadmium accumulating soybean cultivars-Harosoy (high cadmium accumulator), Fukuyutaka (low cadmium accumulator), and their recombinant inbred line CDH-80 (high cadmium accumulator) were exposed to 100 μM CdCl(2) treatment for 3 days. Root growth was found to be affected under cadmium stress in all. Varietal differences at root protein level were evaluated. NADP-dependent alkenal double bond reductase P1 was found to be more abundant in low cadmium accumulating Fukuyutaka. Leaf proteome analysis revealed that differentially expressed proteins were primarily involved in metabolism and energy production. The results indicate that both high and low cadmium accumulating cultivars and CDH-80 share some common defense strategies to cope with the cadmium stress. High abundance of enzymes involved in glycolysis and TCA cycle might help cadmium challenged cells to produce more energy necessary to meet the high energy demand. Moreover, enhanced expressions of photosynthesis related proteins indicate quick utilization of photoassimilates in energy generation. Increased abundance of glutamine synthetase in all might be involved in phytochelatin mediated detoxification of cadmium ions. In addition, increased abundance of antioxidant enzymes, namely superoxide dismutase, ascorbate peroxidase, catalase, ensures cellular protection from reactive oxygen species mediated damages under cadmium stress. Enhanced expression of molecular chaperones in high cadmium accumulating cultivar might be another additional defense mechanism for refolding of misfolded proteins and to stabilize protein structure and function, thus maintain cellular homeostasis.
进行了一项比较蛋白质组学研究，以揭示大豆中镉胁迫反应涉及的蛋白质网络。选用两种具有不同镉积累能力的大豆品种（Harosoy，高镉积累；Fukuyutaka，低镉积累）及其重组自交系 CDH-80（高镉积累）的 10 天大幼苗，用 100μM CdCl2处理 3 天。结果发现所有品种的根生长都受到镉胁迫的影响。评估了根蛋白水平的品种差异。发现 NADP 依赖性烯醛双键还原酶 P1 在低镉积累的 Fukuyutaka 中更为丰富。叶片蛋白质组分析表明，差异表达的蛋白质主要参与代谢和能量产生。结果表明，高镉和低镉积累品种以及 CDH-80 共享一些共同的防御策略来应对镉胁迫。糖酵解和 TCA 循环中参与的酶的高丰度可能有助于镉胁迫细胞产生更多的能量，以满足高能量需求。此外，光合作用相关蛋白的增强表达表明，在能量产生中迅速利用光合作用产物。所有品种谷氨酰胺合成酶的丰度增加可能参与了植物螯合肽介导的镉离子解毒。此外，抗氧化酶（如超氧化物歧化酶、抗坏血酸过氧化物酶、过氧化氢酶）的丰度增加可确保细胞免受镉胁迫下活性氧介导的损伤。高镉积累品种中分子伴侣的增强表达可能是另一种额外的防御机制，用于重折叠错误折叠的蛋白质，并稳定蛋白质结构和功能，从而维持细胞内稳态。
