Fraunhofer Institut für Werkstoff -und Strahltechnik (IWS), Winterbergstrasse 28, 01277, Dresden, Germany.
Biointerphases. 2012 Dec;7(1-4):35. doi: 10.1007/s13758-012-0035-9. Epub 2012 May 15.
Micropatterned surfaces with cell adhesive areas, delimited by protein repellent microstructures, are in high demand for its potential use as relevant biological assays. This is not only because such surfaces allow directing cell growth in a spatially localized and restricted manner, but also because they can be used to elucidate basic cell growth and orientation mechanisms. Here, it is presented a laser-assisted micropatterning technique to fabricate large area microstructures of poly (ethylene glycol) hydrogel onto a cell adhesive surface: a biofunctional maleic anhydride copolymer. By varying photoinitiator, laser intensity, copolymer as well as the hydrogel layer thickness, the optimum conditions to produce high quality features were found. The suitability of these micropatterned substrates for bioassay applications was proved by cell adhesion studies. The introduced procedure could be used to prepare a broad range of microarrays for certain bioanalytical approaches and to create different types of biofunctional surfaces.
具有细胞黏附区域的微图案表面，由蛋白质排斥微结构限定，因其作为相关生物检测的潜在用途而备受需求。这不仅是因为这种表面允许以空间局部和受限的方式引导细胞生长，还因为它们可以用于阐明基本的细胞生长和定向机制。在这里，提出了一种激光辅助微图案化技术，用于在细胞黏附表面：生物功能马来酸酐共聚物上制造聚（乙二醇）水凝胶的大面积微结构。通过改变光引发剂、激光强度、共聚物以及水凝胶层的厚度，找到了产生高质量特征的最佳条件。通过细胞黏附研究证明了这些微图案化基底在生物测定应用中的适用性。所引入的方法可用于制备用于某些生物分析方法的广泛的微阵列，并创建不同类型的生物功能表面。
