College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing, China.
J Sep Sci. 2012 Apr;35(8):1003-9. doi: 10.1002/jssc.201100859.
The paper describes the enhanced separation of o-, m-, p-dihydroxybenzene by capillary electrochromatography (CEC) using gold nanoparticles (AuNPs) as stationary phase. The effect of the AuNPs concentration upon separation was investigated. The experimental parameters, including separation voltage, pH, and concentration of running buffer, were optimized. Under the optimum conditions, a good resolution of three dihydroxybenzene isomers was obtained within 15 min in a 50 cm effective length capillary modified with 0.02 nmol/L AuNPs at a separation voltage of 16 kV in a 50 mmol/L acetate buffer (pH 5.0). The linear ranges were from 10(-6) to 10(-4) mol/L and the detection limits were as low as 10(-7) mol/L. This method was successfully used to analysis two kinds of hair coloring agent sample with recoveries in the range of 90-105% and relative standard deviations (RSD) less than 5.0%.
本文描述了使用金纳米粒子（AuNPs）作为固定相通过胶束电动色谱（CEC）增强对邻苯二酚、间苯二酚和对苯二酚的分离。考察了 AuNPs 浓度对分离的影响。优化了实验参数，包括分离电压、pH 值和运行缓冲液的浓度。在最佳条件下，在 50mmol/L 乙酸盐缓冲液（pH 5.0）中，以 16 kV 的分离电压，在修饰有 0.02 nmol/L AuNPs 的 50cm 有效长度毛细管中，在 15 分钟内可获得三种二羟基苯异构体的良好分离度。线性范围为 10(-6)至 10(-4)mol/L，检测限低至 10(-7)mol/L。该方法成功用于分析两种染发剂样品，回收率在 90-105%范围内，相对标准偏差（RSD）小于 5.0%。
