West Virginia University Eye Institute, West Virginia University, Morgantown, USA.
Invest Ophthalmol Vis Sci. 2012 Jun 22;53(7):3896-905. doi: 10.1167/iovs.11-8762.
Multi-species biofilms associated with contact lens cases and lenses can predispose individuals to contact lens-related inflammatory complications. Our study used culture-independent methods to assess the relationship between the severity of contact lens-related disease and bacteria residing in biofilms of contact lens cases and lenses.
Contact lens cases and lenses from 28 patients referred to the West Virginia University Eye Institute and diagnosed as having mild keratitis, keratitis with focal infiltrates, or corneal ulcers were processed and evaluated for bacterial composition based on 16S ribosomal RNA gene sequencing. Cases and lenses from nine asymptomatic contact lens wearers were processed in a manner similar to controls. Relationships between disease severity, bacterial types, and bacterial diversity were evaluated statistically.
Disease severity and presenting visual acuity correlated with an increase in the diversity of bacterial types isolated from contact lens cases. A significant difference also was observed in the number of bacterial types associated with the three clinical groups. Achromobacter, Stenotrophomonas, and Delftia were prevalent in all disease groups, and Achromobacter and Stenotrophomonas were present in one asymptomatic control. Scanning electron microscopy revealed that Achromobacter and Stenotrophomonas formed a biofilm on the surface of contact lenses.
Culture-independent methods identified an association between disease severity and bacterial diversity in biofilms isolated from cases and lenses of patients with contact lens-related corneal disease. Achromobacter, Stenotrophomonas, and Delftia were predominant bacteria identified in our study, drawing attention to their emerging role in contact lens-related disease.
与隐形眼镜盒和镜片相关的多物种生物膜会使个体易患与隐形眼镜相关的炎症并发症。我们的研究使用非培养方法来评估与接触镜相关疾病的严重程度和存在于接触镜盒和镜片生物膜中的细菌之间的关系。
从因患有轻度角膜炎、局灶性浸润性角膜炎或角膜溃疡而被转诊到西弗吉尼亚大学眼科研究所的 28 名患者中采集隐形眼镜盒和镜片，并基于 16S 核糖体 RNA 基因测序对其进行细菌组成分析。以类似的方式处理来自 9 名无症状隐形眼镜佩戴者的镜片和镜片作为对照。统计评估疾病严重程度、细菌类型和细菌多样性之间的关系。
疾病严重程度和表现视力与从隐形眼镜盒中分离出的细菌类型多样性增加相关。在与三种临床组相关的细菌类型数量上也观察到显著差异。无色杆菌、寡养单胞菌和德氏菌在所有疾病组中都很普遍，而在一个无症状对照组中存在无色杆菌和寡养单胞菌。扫描电子显微镜显示，无色杆菌和寡养单胞菌在隐形眼镜表面形成生物膜。
非培养方法鉴定出与来自患有与隐形眼镜相关的角膜疾病的患者的隐形眼镜盒和镜片中分离出的生物膜中的疾病严重程度和细菌多样性之间存在关联。在我们的研究中，无色杆菌、寡养单胞菌和德氏菌是主要的细菌，这引起了人们对它们在与隐形眼镜相关疾病中作用的关注。
