Nawrath C, Schell J, Koncz C
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Mol Gen Genet. 1990 Aug;223(1):65-75. doi: 10.1007/BF00315798.
Genomic and cDNA clones homologous to the RpII215 gene of Drosophila were isolated from Arabidopsis thaliana and assigned to a single copy gene encoding a transcript of 6.8 kb. Nucleotide sequence analysis of Arabidopsis genomic and cDNAs revealed a striking homology to yeast, Caenorhabditis, Drosophila and mouse genes encoding the largest subunit of RNA polymerase II. The Arabidopsis gene rpII215 contains 13 introns, 12 of which interrupt the coding sequence of a protein of 205 kDa. The position of the first intron is conserved between plant and animal genes, while an intron located in the 3' untranslated region of the rpII215 gene is unique to Arabidopsis. Common domains present in all known largest subunits of eucaryotic RNA polymerase II were identified in the predicted sequence of the Arabidopsis RpII215 protein. Both the order and the position of N-terminal Zn2+ finger and of DNA and alpha-amanitin binding motifs are conserved in Arabidopsis. The C-terminal region of the Arabidopsis protein contains 15 consensus and 26 variant YSPTSPS repeats (CTDs). Highly conserved structure among the various C-terminal domains suggests that the largest subunit of RNA polymerase II in plants may also interact with transcription factors and with protein kinases that control the cell cycle as in other organisms.
从拟南芥中分离出与果蝇RpII215基因同源的基因组和cDNA克隆，并将其定位到一个单拷贝基因，该基因编码一个6.8 kb的转录本。对拟南芥基因组和cDNA的核苷酸序列分析表明，它与酵母、秀丽隐杆线虫、果蝇和小鼠中编码RNA聚合酶II最大亚基的基因具有显著的同源性。拟南芥基因rpII215包含13个内含子，其中12个打断了一个205 kDa蛋白质的编码序列。第一个内含子的位置在植物和动物基因之间是保守的，而位于rpII215基因3'非翻译区的一个内含子是拟南芥特有的。在拟南芥RpII215蛋白的预测序列中鉴定出了所有已知的真核RNA聚合酶II最大亚基中存在的共同结构域。拟南芥中N端锌指、DNA和α-鹅膏蕈碱结合基序的顺序和位置都是保守的。拟南芥蛋白的C端区域包含15个共有和26个变异的YSPTSPS重复序列（CTD）。不同C端结构域之间高度保守的结构表明，植物中RNA聚合酶II的最大亚基可能也像其他生物体一样与转录因子以及控制细胞周期的蛋白激酶相互作用。
