Department of Medical Biochemistry, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Cell Immunol. 2012 Mar-Apr;276(1-2):110-3. doi: 10.1016/j.cellimm.2012.04.009. Epub 2012 Apr 21.
In hepatitis C infection, the production of inappropriate cytokines levels may contribute to viral persistence and may affect the response to antiviral therapy. We investigate the effect of IL4 C-590T and C-33T polymorphisms on the response to combination therapy with interferon and ribavirin in chronic HCV patients. These single nucleotide polymorphisms were determined by PCR-RFLP in 235 responder and 210 non-responder to combination therapy. The IL4-590 T/T and -33 T/T genotypes were associated with resistance to the therapy (p<0.001, p=0.001 respectively). Haplotypes T(-590) T(-33) and T(-590) C(-33) were associated with a higher risk in non-responder patients than the responders (p<0.001 for each) while frequency of haplotype C(-590) C(-33) (with all wild alleles) was significantly higher in responders as compared to non-responders (p<0.001). These results suggest that inheritance of the IL4 polymorphisms may be associated with resistance to combined antiviral therapy in Egyptian HCV patients.
在丙型肝炎感染中，不合适的细胞因子水平的产生可能有助于病毒的持续存在，并可能影响对抗病毒治疗的反应。我们研究了白细胞介素 4（IL4）C-590T 和 C-33T 多态性对慢性丙型肝炎病毒（HCV）患者联合干扰素和利巴韦林治疗反应的影响。这些单核苷酸多态性通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）在 235 名应答者和 210 名无应答者中确定。IL4-590 T/T 和-33 T/T 基因型与治疗耐药性相关（p<0.001，p=0.001）。T(-590) T(-33)和 T(-590) C(-33)的单体型与无应答者的更高风险相关（p<0.001），而具有所有野生等位基因的 C(-590) C(-33)单体型的频率在应答者中明显高于无应答者（p<0.001）。这些结果表明，IL4 多态性的遗传可能与埃及 HCV 患者联合抗病毒治疗的耐药性有关。
