Department of Stomatology, Nanfang Hospital, and College of Stomatology, Southern Medical University, Guangzhou, China.
J Endod. 2012 Jun;38(6):796-802. doi: 10.1016/j.joen.2012.02.014. Epub 2012 Mar 22.
It is suggested that dental pulp stem cells are involved in tooth regeneration and play an important role in maintaining pulp homeostasis. Previously, normal dental pulps were more widely used for experimental models than carious dental pulps. The aim of this study was to isolate and culture the dental pulp stem cells from carious and normal teeth and to evaluate stem cell parameters.
Pulp tissues were obtained and dissociated from normal and carious teeth. Single-cell suspensions were seeded into 6-well plates and purified by collecting multiple colonies. Normal dental pulp stem cells (DPSCs) and carious dental pulp stem cells (CDPSCs) were compared for morphologic appearance and for their capacity to differentiate into 3 lineages. Colony-forming and MTT assays, cell cycle analysis, gene expression, and alkaline phosphatase activity were also evaluated.
Stem cells were cultured successfully from normal and carious dental pulps. CDPSCs displayed increased proliferation ability compared with DPSCs. CDPSCs also showed enhanced ALP activity, mineralization ability, and expression of osteogenesis/dentinogenesis-related genes. All cultures differentiated into 3 cell types.
Our data suggest that caries as a local microenvironment should be taken into account when DPSCs are intended to be used for investigations and application. Furthermore, the mechanism of the underlying changes in cell properties requires further study.
有研究表明牙髓干细胞参与牙齿再生，并在维持牙髓内稳态方面发挥着重要作用。此前，正常牙髓比龋坏牙髓更广泛地用于实验模型。本研究旨在从正常和龋坏牙齿中分离和培养牙髓干细胞，并评估其干细胞参数。
从正常和龋坏牙齿中获取牙髓组织并进行分离。将单细胞悬液接种到 6 孔板中，并通过收集多个集落进行纯化。比较正常牙髓干细胞（DPSCs）和龋坏牙髓干细胞（CDPSCs）的形态外观及其向 3 个谱系分化的能力。还评估了集落形成和 MTT 分析、细胞周期分析、基因表达和碱性磷酸酶活性。
成功地从正常和龋坏牙髓中培养出了干细胞。与 DPSCs 相比，CDPSCs 显示出更强的增殖能力。CDPSCs 还表现出增强的碱性磷酸酶活性、矿化能力和骨/牙本质形成相关基因的表达。所有培养物均分化为 3 种细胞类型。
我们的数据表明，在将 DPSCs 用于研究和应用时，应考虑龋坏作为局部微环境的影响。此外，细胞特性变化的潜在机制需要进一步研究。
