Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region, China.
J Endod. 2011 Aug;37(8):1074-80. doi: 10.1016/j.joen.2011.04.004. Epub 2011 May 23.
Dogs are commonly used animal models for regenerative endodontics research. Although several studies have used stem cells isolated from dog teeth to investigate the dentin/pulp regeneration in vivo, less attention has been paid for the characterization of these cells. Therefore, this study aimed to characterize the dental pulp stem cells isolated from dog teeth (cDPSCs) in order to further define the dog as an animal model for regenerative endodontics.
Stem cells were isolated from freshly extracted premolars of 10-month-old Beagles. The isolated cells were investigated for their stem cell properties by analysis of their clonogenic and growth characteristics; expression of mesenchymal stem cell markers; and evaluation of their osteo/odontogenic, adipogenic, and neurogenic potential.
A colony formation assay showed the existence of a clonogenic cell population in cDPSCs isolated. The growth curves revealed a higher proliferation rate of cDPSCs compared with hBMMSCs. cDPSCs expressed mesenchymal stem cell markers STRO-1, CD146, and Nanog. However, they were negative for CD73, CD105, and CD45. cDPSCs were able to differentiate into odontoblast-like cells as shown by increased alkaline phosphatase activity, dentin sialoprotein expression, and formation of mineralized nodules. cDPSCs also showed the neurogenic and adipogenic differentiation potential at a lower level compared with those of hDPSCs and hBMMSCs.
The results of this study confirmed the stem cell properties of cDPSCs at a comparable level to those of hDPSCs and hBMMSCs. Overall, the data presented in this study provide evidence in supportive of using cDPSCs and dogs as an animal model in dental tissue engineering via stem cell-based approaches.
狗通常被用作再生牙髓学研究的动物模型。尽管已有几项研究使用从狗牙中分离出的干细胞来研究体内牙本质/牙髓再生，但对这些细胞的特征研究较少。因此，本研究旨在对从狗牙中分离出的牙髓干细胞（cDPSCs）进行特征分析，以便进一步将狗定义为再生牙髓学的动物模型。
从 10 月龄比格犬的新鲜拔除前磨牙中分离干细胞。通过分析其克隆形成和生长特性、间充质干细胞标志物的表达以及评估其成骨/成牙本质、成脂和成神经潜能，对分离的细胞进行干细胞特性研究。
集落形成实验表明，cDPSCs 中存在克隆形成细胞群。生长曲线显示 cDPSCs 的增殖率高于 hBMMSCs。cDPSCs 表达间充质干细胞标志物 STRO-1、CD146 和 Nanog。然而，它们对 CD73、CD105 和 CD45 呈阴性。cDPSCs 能够分化为成牙本质样细胞，表现为碱性磷酸酶活性增加、牙本质涎蛋白表达和矿化结节形成。与 hDPSCs 和 hBMMSCs 相比，cDPSCs 的成神经和成脂分化潜能较低。
本研究结果证实了 cDPSCs 的干细胞特性与 hDPSCs 和 hBMMSCs 相当。总的来说，本研究提供的证据支持使用 cDPSCs 和狗作为基于干细胞的方法进行牙组织工程的动物模型。
