Soules M R, Tyrey L, Hammond C B
Am J Obstet Gynecol. 1979 Oct 1;135(3):384-92. doi: 10.1016/0002-9378(79)90711-7.
The diagnosis and treatment of trophoblastic disease depend upon the reliable measurement of human chorionic gonadotropin (hCG) in serum or urine. The double-antibody hCG beta-subunit assay for serum hCG combines great sensitivity, specificity, and precision but requires 36 hours to yield results. This investigation sought to increase the efficiency and total assay volume capabilities of our laboratory through the development of rapid hCG assay to augment the slower beta-subunit system. Two separate rapid hCG assays were examined--a double-antibody radioimmunoassay and a radioreceptor assay. The double-antibody assay took 5 hours to complete, while the radioreceptor assay could be completed with 3 1/2 hours. One hundred serum samples from patients with trophoblastic disease were assayed for hCG in the beta-subunit radioimmunoassay and in both rapid assay systems. No differences in results produced by the rapid double-antibody and the hCG beta-subunit assays were noted; however, results from the radioreceptor assay were significantly different (p less than 0.05) from those obtained in the beta-subunit system.
滋养层疾病的诊断和治疗取决于血清或尿液中绒毛膜促性腺激素（hCG）的可靠检测。用于血清hCG的双抗体hCGβ亚基检测法兼具高灵敏度、特异性和精密度，但需要36小时才能得出结果。本研究旨在通过开发快速hCG检测法来提高我们实验室的效率和总检测量能力，以补充较慢的β亚基系统。研究了两种不同的快速hCG检测法——双抗体放射免疫分析法和放射受体分析法。双抗体分析法需5小时完成，而放射受体分析法可在3.5小时内完成。对100份来自滋养层疾病患者的血清样本进行了hCG检测，分别采用β亚基放射免疫分析法和两种快速检测系统。快速双抗体检测法和hCGβ亚基检测法的结果未发现差异；然而，放射受体分析法的结果与β亚基系统的结果存在显著差异（p<0.05）。
