Wu T, Ballon S C, Teng N N
Am J Obstet Gynecol. 1983 Dec 1;147(7):821-5. doi: 10.1016/0002-9378(83)90047-9.
The level of human chorionic gonadotropin (hCG) in series of sera from eight patients with gestational trophoblastic neoplasia was measured by monoclonal antibody and polyclonal antibody radioimmunoassay. A comparative analysis was performed. Three commercially available monoclonal anti-beta-subunit of hCG (beta-hCG) antibodies were evaluated and the most specific and sensitive one was chosen to develop a quantitative beta-hCG radioimmunoassay. beta-hCG radioimmunoassay kits from Nuclear Medical Systems, Inc., and Clinical Assays served as polyclonal antibody assays. Results obtained with the monoclonal antibody radioimmunoassays demonstrated a high degree of correlation (r greater than 0.95, p less than 0.01) with those obtained by the polyclonal antibody techniques; however, the sera from one patient continuously demonstrated a low level of hCG in the monoclonal antibody radioimmunoassay while registering undetectable levels in the polyclonal antibody assays. Although the monoclonal antibody radioimmunoassay appears to be specific and fairly sensitive, the results indicate that, with current technology, there is no special advantage to employing this assay to measure hCG in patients with gestational trophoblastic neoplasia.
采用单克隆抗体和多克隆抗体放射免疫分析法，对8例妊娠滋养细胞肿瘤患者的一系列血清中的人绒毛膜促性腺激素（hCG）水平进行了测定，并进行了对比分析。对三种市售的抗hCGβ亚基（β-hCG）单克隆抗体进行了评估，选择了最特异和敏感的一种来开发定量β-hCG放射免疫分析方法。来自核医学系统公司和临床检验公司的β-hCG放射免疫分析试剂盒用作多克隆抗体分析方法。单克隆抗体放射免疫分析所得结果与多克隆抗体技术所得结果显示出高度相关性（r大于0.95，p小于0.01）；然而，一名患者的血清在单克隆抗体放射免疫分析中持续显示出低水平的hCG，而在多克隆抗体分析中则显示为不可检测水平。虽然单克隆抗体放射免疫分析似乎具有特异性且相当敏感，但结果表明，就目前的技术而言，采用该分析方法来测定妊娠滋养细胞肿瘤患者的hCG并无特别优势。
