AmorePacific R&D Center, Yongin-si, Republic of Korea.
Toxicol Lett. 2012 Jul 20;212(2):198-204. doi: 10.1016/j.toxlet.2012.05.004. Epub 2012 May 15.
Using a human corneal cell line (HCE-T cells) and 2 evaluation criteria, we developed a new alternative method to assess the eye irritation potential of chemicals. We exposed HCE-T cells to different concentrations of 38 chemicals for 1h and measured relative cell viability (RCV) as an endpoint at each concentration. Using the RCV values, we calculated the RCV50. We also exposed HCE-T cells to 3 fixed concentrations of the 38 chemicals (5%, 0.5%, and 0.05%) for 1h and measured the RCV at each concentration. Using the RCV values at 5%, 0.5%, and 0.05%, we developed a new criterion for eye irritation potential (total eye irritation score, TEIS) and estimated the ocular irritancy. We then assessed the correlation of the results of RCV50 and TEIS with those of the Draize rabbit eye irritation. Both the RCV50 and TEIS results exhibited good positive correlations (sensitivity: 80.77%, specificity: 83.33%, and accuracy: 81.58% for TEIS; sensitivity: 73.08-76.92%, specificity: 75.00%, and accuracy: 73.68-76.32% for RCV50). We conclude that the new in vitro model using HCE-T cells is a good alternative evaluation model for the prediction of the eye irritation potential of chemicals.
我们使用人角膜细胞系（HCE-T 细胞）和 2 种评价标准，开发了一种新的替代方法来评估化学品的眼刺激性。我们将 HCE-T 细胞暴露于 38 种不同浓度的化学物质中 1 小时，并在每个浓度下测量相对细胞活力（RCV）作为终点。使用 RCV 值，我们计算了 RCV50。我们还将 HCE-T 细胞暴露于 38 种化学物质的 3 个固定浓度（5％、0.5％和 0.05％）中 1 小时，并在每个浓度下测量 RCV。使用 5％、0.5％和 0.05％的 RCV 值，我们开发了一种新的眼刺激性潜力标准（总眼刺激性评分，TEIS）并估计了眼部刺激性。然后，我们评估了 RCV50 和 TEIS 结果与 Draize 兔眼刺激性结果的相关性。RCV50 和 TEIS 结果均表现出良好的正相关性（TEIS 的灵敏度：80.77％，特异性：83.33％，准确性：81.58％；RCV50 的灵敏度：73.08-76.92％，特异性：75.00％，准确性：73.68-76.32％）。我们得出结论，使用 HCE-T 细胞的新体外模型是预测化学品眼刺激性潜力的良好替代评价模型。
