Schlein Y, Schnur L F, Jacobson R L
Department of Parasitology, Hadassah Medical School, Jerusalem, Israel.
Trans R Soc Trop Med Hyg. 1990 May-Jun;84(3):353-5. doi: 10.1016/0035-9203(90)90315-6.
The effect of Leishmania glycoconjugate in the vector was investigated using Phlebotomus papatasi artificially infected with a Leishmania major strain that this vector does not transmit in nature. Glycoconjugate of the vector-specific strain of L. major was added to the infective meals of some fly groups and the success of infections with or without this substance was compared 4 d later. In the absence of glycoconjugate the parasites survived in 15.6% of the flies, while the addition of 0.5 mg/ml and 2 mg/ml raised the rate of infection to 34.3% and 63.0% respectively. Undigested blood was found in the stomach of 7.8% of the infected flies, whereas following meals with parasites and 2 mg/ml glycoconjugate it was present in 37% of the flies. The results demonstrated that this glycoconjugate increased the viability of the parasites in the unsuitable vector and delayed digestion of the infective meals. In a parallel experiment the glycoconjugate of L. donovani did not affect the survival of the parasites.
利用感染了硕大利什曼原虫（该载体在自然情况下不传播此菌株）的巴氏白蛉，研究了利什曼原虫糖缀合物在载体中的作用。将硕大利什曼原虫载体特异性菌株的糖缀合物添加到部分蝇组的感染性食物中，并在4天后比较添加该物质与未添加该物质时的感染成功率。在没有糖缀合物的情况下，15.6%的蝇体内寄生虫存活，而添加0.5mg/ml和2mg/ml糖缀合物后，感染率分别提高到34.3%和63.0%。在7.8%的受感染蝇的胃中发现未消化的血液，而在喂食含寄生虫和2mg/ml糖缀合物的食物后，37%的蝇胃中有未消化的血液。结果表明，这种糖缀合物提高了寄生虫在不合适载体中的生存能力，并延缓了感染性食物的消化。在一项平行实验中，杜氏利什曼原虫的糖缀合物不影响寄生虫的存活。
