Iwasaki T, Shimizu M, Obata H, Ogata M, Nagata M, Yanai T, Kitagawa H, Sasaki Y
Veterinary Medical Teaching Hospital, Gifu University, Japan.
Vet Pathol. 1996 May;33(3):332-6. doi: 10.1177/030098589603300310.
The effect of substrate on indirect immunofluorescence (IIF) tests for the detection of circulating autoantibodies was studied by examining sera from 14 canine pemphigus foliaceus patients, six sera with non-pemphigus dermatoses and ten normal dog sera against five different substrates from three species. These substrates included bovine esophagus, bovine nose, bovine tongue, monkey esophagus, and canine nose skin. Nine out of 14 (64.3%) sera from patients with canine pemphigus foliaceus showed intercellular space staining by indirect immunofluorescence using bovine esophagus as substrate. However, sera from nonpemphigus dermatoses and normal dog did not react with bovine esophagus. In other substrates, only bovine tongue showed 1/8 (12.5%) positive reaction at the intercellular space by sera from canine pemphigus foliaceus. Dog nose skin showed the intercellular space staining against ten of ten (100%) normal dog serum. Monkey esophagus showed the fluorescent deposit at the intercellular space in four of nine (44.4%) of pemphigus foliacues dog sera, however, four of ten (40%) of normal dog sera revealed nonspecificity intercellular staining. These results indicate that the sensitivity and the specifity of IIF test in canine pemphigus foliaceus depend on the substrate. The best substrate for detecting circulating autoantibody in canine pemphigus foliaceus patients among five different substrates was bovine esophagus because of its sensitivity and high specificity. The diagnosis of canine pemphigus foliaceus should be made on the basis of a combination of clinical signs, histopathology, direct immunofluorescence, and the detection of circulating autoantibody.
通过检测14例犬落叶型天疱疮患者的血清、6例非天疱疮皮肤病患者的血清以及10例正常犬血清，针对来自三个物种的五种不同底物，研究了底物对检测循环自身抗体的间接免疫荧光（IIF）试验的影响。这些底物包括牛食管、牛鼻、牛舌、猴食管和犬鼻皮肤。14例犬落叶型天疱疮患者的血清中有9例（64.3%），以牛食管为底物通过间接免疫荧光显示细胞间间隙染色。然而，非天疱疮皮肤病患者的血清和正常犬血清与牛食管无反应。在其他底物中，只有牛舌在犬落叶型天疱疮患者的血清作用下，在细胞间间隙显示出八分之一（12.5%）的阳性反应。犬鼻皮肤对十例（100%）正常犬血清均显示细胞间间隙染色。猴食管在九例（44.4%）落叶型天疱疮犬血清中的四例中，在细胞间间隙显示荧光沉积，然而，十例（40%）正常犬血清中的四例显示非特异性细胞间染色。这些结果表明，IIF试验在犬落叶型天疱疮中的敏感性和特异性取决于底物。在五种不同底物中，检测犬落叶型天疱疮患者循环自身抗体的最佳底物是牛食管，因为其具有敏感性和高特异性。犬落叶型天疱疮的诊断应基于临床症状、组织病理学、直接免疫荧光以及循环自身抗体的检测相结合。
