Troedsson M, Concha C, Einarsson S, Holmberg O
Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala.
Acta Vet Scand. 1990;31(2):187-92. doi: 10.1186/BF03547561.
From 6 mares with chronic uterine infection, polymorphonuclear neutrophils (PMNs) were obtained from the uterus. In order to recover an adequate number of viable PMNs, 0.1% oyster glycogen was infused into the uterus as a mild irritant 12 h prior to the uterine flushing. Phagocytosis and chemotaxis of the uterine derived PMNs were determined. The supernatant from the uterine flushing was compared with autologous serum for its capacity as an opsonin and chemoattractant. There was a significant increase of both phagocytosis and chemotaxis when autologous serum was used compared with the supernatant from the uterine flushing. This study indicates that autologous serum has a greater opsonizing capacity than uterine secretion from mares with chronic uterine infection. Since all mares conceived following this study, the use of oyster glycogen was not considered to have deleterious effect on the uterine mucosa.
从6匹患有慢性子宫感染的母马子宫中获取多形核中性粒细胞(PMNs)。为了获得足够数量的存活PMNs,在子宫冲洗前12小时,将0.1%的牡蛎糖原作为轻度刺激物注入子宫。测定子宫来源的PMNs的吞噬作用和趋化性。将子宫冲洗液的上清液与自体血清作为调理素和趋化剂的能力进行比较。与子宫冲洗液的上清液相比,使用自体血清时吞噬作用和趋化性均显著增加。本研究表明,自体血清比患有慢性子宫感染的母马的子宫分泌物具有更强的调理能力。由于本研究之后所有母马均受孕,因此认为使用牡蛎糖原对子宫黏膜没有有害影响。