Pycock J F, Allen W E
Department of Surgery and Obstetrics, Royal Veterinary College, Hatfield, Herts, UK.
Tierarztl Prax Suppl. 1989;4:17-20.
The uteri of normal pony mares in oestrus were infected experimentally with Streptococcus zooepidemicus. Uterine contents were collected 30, 60, 120 or 240 minutes later and were tested for their chemotactic effect on equine-neutrophils both with a morphological assay based on neutrophil shape changes and with a modified Boyden chamber technique. By 30 minutes after infection the uterine contents were markedly chemotactic for isolated peripheral neutrophils and remained so at the 240-minute collection. Uterine contents from uninfected mares had minimal chemotactic properties. These results could explain the early neutrophil response to acute endometritis in the mare which probably involves activation of an endogenous chemotactic system.
在发情期，对正常小母马的子宫进行了兽疫链球菌的实验性感染。在感染后30、60、120或240分钟收集子宫内容物，并通过基于中性粒细胞形状变化的形态学检测法和改良的博伊登小室技术，检测其对马中性粒细胞的趋化作用。感染后30分钟，子宫内容物对分离的外周中性粒细胞具有明显的趋化作用，在240分钟收集时仍保持如此。未感染母马的子宫内容物趋化特性极小。这些结果可以解释母马对急性子宫内膜炎的早期中性粒细胞反应，这可能涉及内源性趋化系统的激活。
