Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Korea.
J Fish Dis. 2012 Jul;35(7):481-7. doi: 10.1111/j.1365-2761.2012.01382.x. Epub 2012 May 18.
Lactococcus garvieae is recognized as an emerging pathogen in fish. Several PCR-based methods have been developed for the detection and identification of L. garvieae; however, the sensitivity of these methods is still in question regarding the discrimination of this organism from other closely related species. Two primers, ITSLg30F and ITSLg319R, were designed from the sequence in the 16S-23S internal transcribed spacer (ITS) region and used for the specific detection of L. garvieae. L. garvieae strains including fish isolates were positive by this method. In contrast, previously developed PCR methods showed false-positive results with non-L. garvieae species. Our results indicate that a PCR method using the newly designed ITS primer set provides a sensitive and efficient tool for the detection of L. garvieae in fish and aquaculture environments.
garvieae 乳球菌被认为是鱼类中的一种新兴病原体。已经开发了几种基于 PCR 的方法来检测和鉴定 L. garvieae；然而，这些方法的灵敏度在从其他密切相关的物种区分该生物体方面仍存在疑问。两条引物 ITSLg30F 和 ITSLg319R 是根据 16S-23S 内部转录间隔区 (ITS) 序列设计的，用于特异性检测 L. garvieae。该方法可检测到包括鱼类分离株在内的 L. garvieae 菌株呈阳性。相比之下，先前开发的 PCR 方法在非 L. garvieae 物种中显示出假阳性结果。我们的结果表明，使用新设计的 ITS 引物组的 PCR 方法为检测鱼类和水产养殖环境中的 L. garvieae 提供了一种敏感和高效的工具。
