Barberá-Guillem E, Alonso-Varona A, Boyano M D, Vidal-Vanaclocha F
Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain.
Anat Rec. 1990 Nov;228(3):267-76. doi: 10.1002/ar.1092280306.
Hepatocyte enzyme activity was demonstrated by examining adult C57BL/6 mouse liver cryostat sections under a succinate dehydrogenase (SDH) histochemical reaction, and quantified by microspectrophotometry and microdensitometry. The hepatocyte SDH activity gradient along the path between the portal veins (PV) and efferent terminal hepatic venules (THV) was analyzed by measuring the concentration of the chromophore precipitated in 10 consecutive hepatic parenchymal domains located along imaginary lines drawn across the entire PV-to-THV distance. The profiles of intensity or of normalized relative optical density obtained on a high number of lines were correlated with distance values along the PV-to-THV pathway, enabling us to establish a general mathematical function relating SDH activity (chromophore concentration) to position values on a scale of 0 to 10 corresponding to the theoretical PV-to-THV distance. The equation can be used to interpolate the SDH activity surrounding any intrahepatic object located between the PV and the THV, thus making it possible to calculate the object's anatomical-functional position coordinates in the liver acinus. To demonstrate how this method is used, we have calibrated the intrahepatic position of hemopoietic foci induced in the liver tissue of adult mice treated with phenylhydrazine (PHZ), and show that these foci are located on coordinate 3.31 (maximum range 1.25-4.86) of the sinusoidal domain-that is, on the borderline between Rappaport's acinar zones 1 and 2.
通过在琥珀酸脱氢酶（SDH）组织化学反应下检查成年C57BL/6小鼠肝脏冷冻切片来证明肝细胞酶活性，并通过显微分光光度法和显微密度测定法进行定量。通过测量沿着横跨门静脉（PV）到传出终末肝静脉（THV）的整个距离所绘制的假想线上的10个连续肝实质区域中沉淀的发色团浓度，分析肝细胞SDH活性沿门静脉（PV）和传出终末肝静脉（THV）之间路径的梯度。在大量线上获得的强度或归一化相对光密度的分布与沿PV到THV途径的距离值相关，使我们能够建立一个通用的数学函数，将SDH活性（发色团浓度）与对应于理论PV到THV距离的0到10尺度上的位置值相关联。该方程可用于内插位于PV和THV之间的任何肝内物体周围的SDH活性，从而能够计算该物体在肝腺泡中的解剖功能位置坐标。为了说明如何使用这种方法，我们校准了用苯肼（PHZ）处理的成年小鼠肝脏组织中诱导的造血灶的肝内位置，并表明这些灶位于正弦域的坐标3.31（最大范围1.25 - 4.86）处，即位于拉帕波特腺泡区1和2的边界上。
