Barberá-Guillem E, Ayala R, Vidal-Vanaclocha F
Department of Cellular Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Vizcaya, Spain.
Hepatology. 1989 Jan;9(1):29-36. doi: 10.1002/hep.1840090106.
We have measured the location of embryonic and adult hemopoietic foci in the liver tissue of postnatal and adult phenylhydrazine-treated mice. Differentiation of acinar domains in liver tissue was made possible by carrying out succinate dehydrogenase histochemical reactions on liver cryostat sections. To determine the position of hemopoietic foci within the lobular gradient of the hepatocyte succinate dehydrogenase activity, this enzyme was measured in hepatocytes surrounding both portal and central veins and hemopoietic foci. Then, assuming the periportal succinate dehydrogenase activity value to be 1.00 +/- 0.2, succinate dehydrogenase activity around postnatal hemopoietic foci was 0.65 +/- 0.19, around phenylhydrazine-induced hemopoietic foci 0.83 +/- 0.24 and around central veins 0.44 +/- 0.11. Scaling the portal to central vein distance and taking 1 as the portal vein point and 0 as the central vein point, the relative position of hemopoietic foci, indirectly calculated from succinate dehydrogenase activity values, was 0.35 +/- 0.13 in postnatal livers and 0.73 +/- 0.12 in phenylhydrazine-treated adult livers. Hemopoietic foci frequencies varied according to both the origin and the liver acinar domain: in postnatal liver acini, it was 37.1% in zone 1, 22.8% in zone 2 and 40% in zone 3; in phenylhydrazine-treated adult acini, it was 89.4% in zone 1 and 10.6% in zone 2. Postnatal hemopoietic foci mainly occurred extrasinusoidally, between hepatocytes and reticular-like cells, whereas adult hemopoietic foci were mostly intrasinusoidal and closely associated to macrophage-like cells. Adult hemopoietic colonies
我们测量了出生后及成年期经苯肼处理的小鼠肝脏组织中胚胎期和成年期造血灶的位置。通过对肝脏冰冻切片进行琥珀酸脱氢酶组织化学反应，得以区分肝脏组织中的腺泡区域。为了确定造血灶在肝细胞琥珀酸脱氢酶活性小叶梯度内的位置，我们测定了门静脉和中央静脉周围以及造血灶周围肝细胞中的该酶活性。然后，假设门静脉周围琥珀酸脱氢酶活性值为1.00±0.2，出生后造血灶周围的琥珀酸脱氢酶活性为0.65±0.19，苯肼诱导的造血灶周围为0.83±0.24，中央静脉周围为0.44±0.11。将门静脉到中央静脉的距离按比例缩放，以门静脉点为1，中央静脉点为0，根据琥珀酸脱氢酶活性值间接计算出的造血灶相对位置，在出生后肝脏中为0.35±0.13，在苯肼处理的成年肝脏中为0.73±0.12。造血灶频率因起源和肝脏腺泡区域而异：在出生后肝脏腺泡中，1区为37.1%，2区为22.8%，3区为40%；在苯肼处理的成年腺泡中，1区为89.4%，2区为10.6%。出生后造血灶主要发生在肝窦外，位于肝细胞和网状样细胞之间，而成年造血灶大多位于肝窦内，且与巨噬细胞样细胞密切相关。成年造血集落
