Luis Razetti Institute of Oncology and Clínica Santa Sofía, Caracas, Venezuela.
Cancer Epidemiol. 2012 Oct;36(5):e284-7. doi: 10.1016/j.canep.2012.04.005. Epub 2012 May 17.
This study investigated the distribution of human papillomavirus (HPV) types in invasive cervical cancer (ICC), cervical intraepithelial neoplasia 2 (CIN2) and cervical intraepithelial neoplasia 3 (CIN3) in Venezuela.
Paraffin-embedded samples from 329 women from 29 medical centers of the 24 states of Venezuela were analyzed to determine the distribution of HPV types for ICC, CIN2, and CIN3, the prevalence of single and multiple infection, and the association of HPV types with severity of lesion, comparing CIN2 versus CIN3+ (CIN3 and ICC). The samples were analyzed with the polymerase chain reaction (PCR) followed by reverse hybridization for the identification of HPV types.
HPV was identified in 95/96 ICC specimens (98.9%), in 142/149 CIN3 (95.3%) and in 78/84 CIN2 samples (92.8%). The most common types for ICC and CIN3 were: HPV16, 18, 31, and 33, and for CIN2 were HPV16, 31, 51, 52, and 18. HPV single infection was found in 82.1% of ICC cases, in 79.4% of CIN2 cases, and in 77.4% of CIN3 cases. HPV16 was identified as a single infection more frequently in women with CIN3+ than in those with CIN2 (68.6% versus 46.7%, P=0.002), and HPV16 or HPV18 types were more prevalent in CIN3+ than in CIN2 (73.4% versus 50%, P=0.0006).
this is the first study of the distribution of HPV types in ICC, CIN2, and CIN3 conducted throughout the territory of Venezuela. HPV16 and HPV18 were the most frequent HPV types identified in single and multiple infections in both ICC and CIN3 groups, and are associated with severity of lesion. The knowledge of the distribution of HPV types would allow organization of an HPV-DNA-based screening test, and consideration of the implementation of prophylactic vaccination in Venezuela.
本研究旨在调查委内瑞拉浸润性宫颈癌（ICC）、宫颈上皮内瘤变 2 级（CIN2）和宫颈上皮内瘤变 3 级（CIN3）中人类乳头瘤病毒（HPV）型别的分布情况。
对来自委内瑞拉 29 家医疗中心的 329 名女性的石蜡包埋样本进行分析，以确定 ICC、CIN2 和 CIN3 中 HPV 型别的分布情况、单一和多重感染的流行情况，以及 HPV 型别与病变严重程度的关系，并将 CIN2 与 CIN3+（CIN3 和 ICC）进行比较。采用聚合酶链反应（PCR）结合反向杂交技术对 HPV 型别进行鉴定。
96 例 ICC 标本中 95 例（98.9%）、149 例 CIN3 标本中 142 例（95.3%）和 84 例 CIN2 标本中 78 例（92.8%）均检出 HPV。ICC 和 CIN3 最常见的类型为 HPV16、18、31 和 33，CIN2 为 HPV16、31、51、52 和 18。82.1%的 ICC 病例、79.4%的 CIN2 病例和 77.4%的 CIN3 病例为 HPV 单一感染。与 CIN2 相比，CIN3+中 HPV16 单一感染更为常见（68.6%比 46.7%，P=0.002），CIN3+中 HPV16 或 HPV18 型更为常见（73.4%比 50%，P=0.0006）。
这是委内瑞拉首次对 ICC、CIN2 和 CIN3 中 HPV 型别的分布情况进行的研究。HPV16 和 HPV18 是 ICC 和 CIN3 组中单一和多重感染最常见的 HPV 类型，与病变严重程度相关。了解 HPV 型别的分布情况将有助于组织 HPV-DNA 筛查试验，并考虑在委内瑞拉实施预防性疫苗接种。
