Department of Food Hygiene and Food Technology, Veterinary Faculty, University of León, Campus de Vegazana, s/n, 24071 León, Spain.
Food Microbiol. 2012 Sep;31(2):173-80. doi: 10.1016/j.fm.2012.03.012. Epub 2012 Apr 3.
The influence of the serotype on the growth behaviour and the ability to form biofilms of Salmonella enterica strains was investigated. The relationships between biofilm formation and growth kinetic parameters were also determined. A total of 69 strains (61 isolates from poultry and 8 reference strains from culture collections) belonging to 10 serotypes (S. enterica serotype Typhimurium, S. Newport, S. Paratyphi B, S. Poona, S. Derby, S. Infantis, S. Enteritidis, S. Virchow, S. Agona and S. Typhi) were tested. All Salmonella strains produced biofilms on polystyrene micro-well plates (crystal violet assay). Isolates were classified as weak (35 strains), moderate (22), or strong (12) biofilm producers. S. Agona and S. Typhi produced the most substantial (P < 0.001) biofilms. Growth curves were performed at 37 °C in tryptone soy broth by means of optical density (OD(420-580)) measurements from 0 to 48 h. Growth kinetic parameters (Gompertz model) varied between serotypes. The maximum growth rate (ΔOD(420-580)/h) ranged from 0.030 ± 0.002 (S. Typhi) to 0.114 ± 0.011 (S. Agona). The ability of Salmonella strains to form biofilms was not related to their growth kinetic parameters. The formation of biofilms by Salmonella on polystyrene constitutes an issue of concern because plastic materials are frequently used in food facilities. The findings suggest that special efforts must be made for the effective control of Salmonella in food-processing environments when S. Agona or S. Typhi strains are present.
本研究调查了血清型对肠炎沙门氏菌菌株生长行为和生物膜形成能力的影响，并确定了生物膜形成与生长动力学参数之间的关系。共检测了 69 株菌（61 株来自禽源，8 株来自培养物收集），分属于 10 个血清型（肠炎沙门氏菌血清型 Typhimurium、Newport、Paratyphi B、Poona、Derby、Infantis、Enteritidis、Virchow、Agona 和 Typhi）。所有肠炎沙门氏菌菌株均在聚苯乙烯微孔板上形成生物膜（结晶紫测定法）。分离株被分为弱（35 株）、中（22 株）或强（12 株）生物膜生产者。Agona 和 Typhi 产生的生物膜最多（P<0.001）。在 37°C 的胰蛋白胨大豆肉汤中通过 OD（420-580）值（0 至 48 小时）进行生长曲线。生长动力学参数（Gompertz 模型）在血清型之间存在差异。最大生长率（ΔOD（420-580）/h）范围从 0.030±0.002（Typhi）到 0.114±0.011（Agona）。沙门氏菌菌株形成生物膜的能力与它们的生长动力学参数无关。沙门氏菌在聚苯乙烯上形成生物膜是一个令人关注的问题，因为塑料材料经常用于食品设施。研究结果表明，当存在 Agona 或 Typhi 菌株时，必须特别努力有效地控制食品加工环境中的沙门氏菌。
