Wang Hui, Shen Jiawei, Zhu Chengfeng, Ma Kai, Fang Mengcheng, Li Bingbing, Wang Wenhui, Xue Ting
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Food Procession Research Institute, Anhui Agricultural University, Hefei 230036, China.
Foods. 2022 Jul 22;11(15):2185. doi: 10.3390/foods11152185.
Handmade dairy products, which retain the nutrients in milk to the greatest extent, have become popular in China recently. However, no investigation regarding the characteristics of () in raw milk of handmade dairy retail stores has been reported. Here, we investigated the antimicrobial susceptibility, virulence, biofilm formation, and genetic diversity of in raw milk from handmade dairy retail stores in Hefei, China. After 10 months of long-term monitoring, 50 strains were isolated from 69 different raw milk samples, of which 6 were positive for methicillin-resistant (MRSA). The resistance rates of these isolates to ampicillin, erythromycin, kanamycin, tetracycline, sulfamethoxazole-trimethoprim, gentamicin, ofloxacin, oxacillin, chloramphenicol, and doxycycline were 56, 54, 40, 24, 22, 22, 18, 14, 8 and 6%, respectively. All 50 isolates were susceptible to vancomycin and 29 strains (58%) showed multidrug resistance phenotype. For enterotoxins genes, (14%) was detected the most frequently, followed by (6%), (4%), (4%), (4%), (4%), and (2%). By microplate assay, 32 and 68% of the strains showed moderate and strong biofilm formation ability, respectively. Fifty isolates were discriminated into nine types, and the most common typing was t034 (42%). The results of this study indicate that from raw milk may constitute a risk concerning food poisoning, and more attention must be given to awareness and hygienic measures in the food industry.
手工乳制品最大程度地保留了牛奶中的营养成分，近来在中国颇受欢迎。然而，尚无关于手工乳制品零售商店原料奶中（）特性的调查报道。在此，我们对中国合肥手工乳制品零售商店原料奶中的抗菌药敏性、毒力、生物膜形成及基因多样性进行了调查。经过10个月的长期监测，从69份不同的原料奶样品中分离出50株（）菌株，其中6株对耐甲氧西林（MRSA）呈阳性。这些分离株对氨苄西林、红霉素、卡那霉素、四环素、磺胺甲恶唑-甲氧苄啶、庆大霉素、氧氟沙星、苯唑西林、氯霉素和强力霉素的耐药率分别为56%、54%、40%、24%、22%、22%、18%、14%、8%和6%。所有50株分离株对万古霉素敏感，29株（58%）表现出多重耐药表型。对于肠毒素基因，（14%）检测频率最高，其次是（6%）、（4%）、（4%）、（4%）、（4%）和（2%）。通过微孔板试验，分别有32%和68%的菌株表现出中度和强生物膜形成能力。50株分离株被分为9种（）类型，最常见的（）分型是t034（42%）。本研究结果表明，原料奶中的（）可能构成食物中毒风险，食品行业必须更加重视提高认识和采取卫生措施。
