Farias Mirelle S, Budni Patrícia, Ribeiro Carine M, Parisotto Eduardo B, Santos Carla Eliete I, Dias Johnny Ferraz, Dalmarco Eduardo M, Fröde Tânia Silvia, Pedrosa Rozangela C, Wilhelm Filho Danilo
Departamento de Ecologia e Zoologia, Universidade Federal de Santa Catarina, Brazil.
Gastroenterol Hepatol. 2012 Jun-Jul;35(6):386-94. doi: 10.1016/j.gastrohep.2012.03.004. Epub 2012 May 17.
Reactive oxygen species (ROS) overgeneration is involved in the pathogenesis of hepatitis C. The aim of this study was to evaluate the antioxidant status in the blood of HCV infected patients treated or not with standard therapy before and after supplementation of vitamins E, C and zinc. Biomarkers of oxidative stress were evaluated in the blood of three groups of patients: group 1 - controls; group 2 - HCV patients without treatment examined before and after a daily antioxidant supplementation (vitamin E 800 mg, C 500 mg and zinc 40 mg) for 6 months; and group 3 - HCV patients treated with pegylated interferon combined with ribavirin, also examined before and after the same antioxidant supplementation. Before antiviral treatment HCV patients showed enhanced superoxide dismutase, catalase and glutathione peroxidase activities and decreased glutathione reductase activity, while lipoperoxidation was increased and reduced glutathione showed decreased levels compared to controls. Treatment with standard therapy enhanced the activities of catalase and glutathione S-transferase, increased contents of protein carbonyl and promoted further reduced glutathione depletion. After antioxidant supplementation, decreased catalase and glutathione S-transferase activities, decreased lipoperoxidation in group 2, and increased reduced glutathione contents in both supplemented groups were detected. Before antioxidant supplementation, alanine aminotransferase and gamma glutamyl transferase contents showed significant increases in group 2.
Untreated HCV patients and also those treated with the standard therapy are coping with a systemic oxidative stress. The antioxidant supplementation conferred an antioxidant protection to both supplemented groups attenuating oxidation processes related to the disease.
活性氧（ROS）过量生成参与丙型肝炎的发病机制。本研究旨在评估丙型肝炎病毒（HCV）感染患者在补充维生素E、C和锌之前和之后，接受或未接受标准治疗的血液中的抗氧化状态。在三组患者的血液中评估氧化应激生物标志物：第1组 - 对照组；第2组 - 未接受治疗的HCV患者，在每日补充抗氧化剂（维生素E 800毫克、C 500毫克和锌40毫克）6个月前后进行检查；第3组 - 接受聚乙二醇化干扰素联合利巴韦林治疗的HCV患者，同样在补充相同抗氧化剂前后进行检查。在抗病毒治疗前，HCV患者的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性增强，而谷胱甘肽还原酶活性降低，与对照组相比，脂质过氧化增加且还原型谷胱甘肽水平降低。标准治疗增强了过氧化氢酶和谷胱甘肽S-转移酶的活性，增加了蛋白质羰基含量，并进一步促进了还原型谷胱甘肽的消耗。抗氧化剂补充后，检测到第2组的过氧化氢酶和谷胱甘肽S-转移酶活性降低、脂质过氧化减少，且两个补充组的还原型谷胱甘肽含量均增加。在抗氧化剂补充前，第2组的丙氨酸转氨酶和γ-谷氨酰转移酶含量显著增加。
未经治疗的HCV患者以及接受标准治疗的患者都在应对全身性氧化应激。抗氧化剂补充为两个补充组提供了抗氧化保护，减轻了与疾病相关的氧化过程。
