Research Institute for Microbial Diseases, Osaka University, Suita City, Osaka, Japan.
Fertil Steril. 2012 Aug;98(2):465-70. doi: 10.1016/j.fertnstert.2012.04.029. Epub 2012 May 19.
To determine the physiological role of IGSF8 for fertility.
Experimental prospective study.
Academic basic research laboratory.
ANIMAL(S): C57BL/6J and hybrid B6D2F1 mice, as well as Cd9 and Igsf8 knockout mice (C57BL/6J and 129/SvJ mix background), were used for this study.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): In vitro and in vivo fertility tests of Igsf8 knockout mice.
RESULT(S): Tetraspanin family member CD9 plays an important role in sperm-egg fusion. Recently, some researchers have reported that CD9 tightly associates with the immunoglobulin superfamily member IGSF8 on the egg surface and that IGSF8 is undetectable on the surface of Cd9-deficient eggs. This led us to hypothesize that IGSF8 participates in sperm-egg fusion together with CD9. To examine the physiological role of IGSF8 in vivo, we generated Igsf8-deficient mice by homologous recombination and examined the fertility of the females.
CONCLUSION(S): The Igsf8-deficient female mice showed no fertilization defect in vitro or in vivo. We observed that Igsf8-deficient eggs retained the normal level and localization of CD9, resulting in normal microvilli formation, which indicates that IGSF8 is dispensable in fertility.
确定 IGSF8 对生育能力的生理作用。
实验前瞻性研究。
学术基础研究实验室。
C57BL/6J 和杂交 B6D2F1 小鼠，以及 Cd9 和 Igsf8 敲除小鼠（C57BL/6J 和 129/SvJ 混合背景），用于本研究。
无。
IGSF8 敲除小鼠的体内外生育能力测试。
四跨膜蛋白家族成员 CD9 在精卵融合中起重要作用。最近，一些研究人员报告说，CD9 与卵表面的免疫球蛋白超家族成员 IGSF8 紧密结合，而 Cd9 缺陷卵表面检测不到 IGSF8。这使我们假设 IGSF8 与 CD9 一起参与精卵融合。为了在体内检查 IGSF8 的生理作用，我们通过同源重组生成了 Igsf8 敲除小鼠，并检查了雌性的生育能力。
Igsf8 敲除的雌性小鼠在体内和体外均未显示出受精缺陷。我们观察到 Igsf8 敲除的卵保留了正常水平和定位的 CD9，导致正常的微绒毛形成，这表明 IGSF8 在生育能力中是可有可无的。
