Proteomics Unit, Biomedical Research Center (CIB), Navarra Health Service, 31008 Pamplona, Spain.
J Proteomics. 2012 Jul 16;75(13):4005-16. doi: 10.1016/j.jprot.2012.05.011. Epub 2012 May 15.
The olfactory bulb (OB) is the first site for the processing of olfactory information in the brain and its deregulation is associated with neurodegenerative disorders. Although different efforts have been made to characterize the human brain proteome in depth, the protein composition of the human OB remains largely unexplored. We have performed a comprehensive analysis of the human OB proteome employing protein and peptide fractionation methods followed by LC-MS/MS, identifying 1529 protein species, corresponding to 1466 unique proteins, which represents a 7-fold increase in proteome coverage with respect to previous OB proteome descriptions from translational models. Bioinformatic analyses revealed that protein components of the OB participated in a plethora of biological process highlighting hydrolase and phosphatase activities and nucleotide and RNA binding activities. Interestingly, 631 OB proteins identified were not previously described in protein datasets derived from large-scale Human Brain Proteome Project (HBPP) studies. In particular, a subset of these differential proteins was mainly involved in axon guidance, opioid signaling, neurotransmitter receptor binding, and synaptic plasticity. Taken together, these results increase our knowledge about the molecular composition of the human OB and may be useful to understand the molecular basis of the olfactory system and the etiology of its disorders.
嗅球（OB）是大脑中处理嗅觉信息的第一个部位，其功能失调与神经退行性疾病有关。尽管已经做出了不同的努力来深入描述人类大脑蛋白质组，但人类 OB 的蛋白质组成在很大程度上仍未被探索。我们采用蛋白质和肽分级方法，随后进行 LC-MS/MS，对人类 OB 蛋白质组进行了全面分析，鉴定出 1529 种蛋白质，对应于 1466 种独特蛋白质，与之前从转化模型描述的 OB 蛋白质组相比，蛋白质组覆盖率增加了 7 倍。生物信息学分析表明，OB 的蛋白质成分参与了大量的生物学过程，突出了水解酶和磷酸酶活性以及核苷酸和 RNA 结合活性。有趣的是，在源自大型人类大脑蛋白质组计划（HBPP）研究的蛋白质数据集，并未描述 631 种 OB 蛋白质。特别是，这些差异蛋白质的一部分主要涉及轴突导向、阿片样物质信号、神经递质受体结合和突触可塑性。总之，这些结果增加了我们对人类 OB 分子组成的了解，可能有助于理解嗅觉系统的分子基础及其疾病的病因。
