Department of Chemistry, Washington State University, Pullman, WA 99163, USA.
Free Radic Biol Med. 2012 Aug 1;53(3):508-20. doi: 10.1016/j.freeradbiomed.2012.05.008. Epub 2012 May 15.
Current viewpoints concerning the bactericidal mechanisms of neutrophils are reviewed from a perspective that emphasizes challenges presented by the inability to duplicate ex vivo the intracellular milieu. Among the challenges considered are the influences of confinement upon substrate availability and reaction dynamics, direct and indirect synergistic interactions between individual toxins, and bacterial responses to stressors. Approaches to gauging relative contributions of various oxidative and nonoxidative toxins within neutrophils using bacteria and bacterial mimics as intrinsic probes are also discussed.
目前，从强调无法在体外复制细胞内环境的角度出发，对中性粒细胞杀菌机制的现有观点进行了综述。所考虑的挑战包括限制对底物可用性和反应动力学的影响、单个毒素之间的直接和间接协同作用以及细菌对胁迫的反应。还讨论了使用细菌和细菌模拟物作为内在探针来评估中性粒细胞中各种氧化和非氧化毒素的相对贡献的方法。
