Departamento de Biologia, Universidade Federal de Lavras, Campus UFLA, Lavras, Minas Gerais, Brazil.
Syst Appl Microbiol. 2012 Jun;35(4):253-62. doi: 10.1016/j.syapm.2012.04.001. Epub 2012 May 18.
Forty-eight Burkholderia isolates from different land use systems in the Amazon region were compared to type strains of Burkholderia species for phenotypic and functional characteristics that can be used to promote plant growth. Most of these isolates (n=46) were obtained by using siratro (Macroptilium atropurpureum - 44) and common bean (Phaseolus vulgaris - 2) as the trap plant species; two isolates were obtained from nodules collected in the field from Indigofera suffruticosa and Pithecellobium sp. The evaluated characteristics were the following: colony characterisation on "79" medium, assimilation of different carbon sources, enzymatic activities, solubilisation of phosphates, nitrogenase activity and antifungal activity against Fusarium oxysporium f. sp. phaseoli. Whole cell protein profiles, 16S rRNA, gyrB, and recA gene sequencing and multilocus sequence typing were used to identify the isolates. The isolates showed different cultural and biochemical characteristics depending on the legume species from which they were obtained. Except for one isolate from I. suffruticosa, all isolates were able to solubilise calcium phosphate and present nitrogenase activity under free-living conditions. Only one isolate from common beans, showed antifungal activity. The forty four isolates from siratro nodules were identified as B. fungorum; isolates UFLA02-27 and UFLA02-28, obtained from common bean plants, were identified as B. contaminans; isolate INPA89A, isolated from Indigofera suffruticosa, was a close relative of B. caribensis but could not be assigned to an established species; isolate INPA42B, isolated from Pithecellobium sp., was identified as B. lata. This is the first report of nitrogenase activity in B. fungorum, B. lata and B. contaminans.
从亚马逊地区不同土地利用系统中分离的 48 株伯克霍尔德氏菌与伯克霍尔德氏菌属的模式菌株进行了比较，以确定可用于促进植物生长的表型和功能特征。这些分离株中的大多数（n=46）是通过使用银叶豇豆（Macroptilium atropurpureum-44）和普通菜豆（Phaseolus vulgaris-2）作为诱捕植物物种获得的；有两个分离株是从 Indigofera suffruticosa 和 Pithecellobium sp. 收集的根瘤中获得的。评估的特征如下：在“79”培养基上的菌落特征、不同碳源的同化、酶活性、磷酸盐的溶解、固氮酶活性和对尖孢镰刀菌 f. sp. phaseoli 的抗真菌活性。使用全细胞蛋白图谱、16S rRNA、gyrB 和 recA 基因测序和多位点序列分型来鉴定分离株。根据分离株的豆科植物来源，分离株表现出不同的培养和生化特征。除了从 Indigofera suffruticosa 中分离出的一个分离株外，所有分离株都能够溶解磷酸钙并在自由生活条件下表现出固氮酶活性。只有来自普通菜豆的一个分离株表现出抗真菌活性。来自银叶豇豆根瘤的 44 个分离株被鉴定为 B. fungorum；从普通菜豆植物中分离出的 UFLA02-27 和 UFLA02-28 分离株被鉴定为 B. contaminans；从 Indigofera suffruticosa 中分离出的 INPA89A 分离株与 B. caribensis 密切相关，但不能归为已建立的物种；从 Pithecellobium sp. 中分离出的 INPA42B 分离株被鉴定为 B. lata。这是首次报道 B. fungorum、B. lata 和 B. contaminans 具有固氮酶活性。
