Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Quality Safety Centre of Agricultural Products, Shanghai, China.
BMC Microbiol. 2019 Feb 11;19(1):36. doi: 10.1186/s12866-019-1411-0.
A major facilitator superfamily transporter Dehp2 was recently shown to be playing an important role in transport and biodegradation of haloacids in Paraburkholderia caribensis MBA4, and Dehp2 is phylogenetically conserved in Burkholderia sensu lato.
We designed both Burkholderia sensu stricto-specific and Paraburkholderia-specific qPCR assays based on dehp2 and 16S rRNA, and validated the qPCR assays in 12 bacterial strains. The qPCR assays could detect single species of Burkholderia sensu stricto or Paraburkholderia with high sensitivity and discriminate them in mixtures with high specificity over a wide dynamic range of relative concentrations. At relatively lower cost compared with sequencing-based approach, the qPCR assays will facilitate discrimination of Burkholderia sensu stricto and Paraburkholderia in a large number of samples.
For the first time, we report the utilization of a haloacids transporter gene for discriminative purpose in Burkholderia sensu lato. This enables not only quick decision on proper handling of putative pathogenic samples in Burkholderia sensu stricto group but also future exploitation of relevant species in Paraburkholderia group for haloacids biodegradation purposes.
最近的研究表明，主要易化超家族转运蛋白 Dehp2 在 Paraburkholderia caribensis MBA4 中 haloacids 的运输和生物降解中发挥着重要作用，并且 Dehp2 在 Burkholderia 属中具有系统发育保守性。
我们基于 dehp2 和 16S rRNA 设计了 Burkholderia 属特异性和 Paraburkholderia 属特异性 qPCR 检测方法，并在 12 株细菌菌株中验证了这些 qPCR 检测方法。qPCR 检测方法具有较高的灵敏度，可检测到 Burkholderia 属或 Paraburkholderia 属的单一种群，特异性高，在相对浓度的宽动态范围内可对混合物进行区分。与基于测序的方法相比，qPCR 检测方法成本相对较低，将有助于在大量样品中区分 Burkholderia 属和 Paraburkholderia 属。
我们首次报告了 haloacids 转运蛋白基因在 Burkholderia 属中的鉴别用途。这不仅可以快速决定 Burkholderia 属中疑似致病样本的适当处理方式，还可以为 Paraburkholderia 属中 haloacids 生物降解相关物种的未来开发提供依据。
