Laboratory for DNA Analysis and Molecular Diagnostics, Department of Nephrology, Medical University of Lublin, Lublin, Poland.
Hum Immunol. 2012 Aug;73(8):824-8. doi: 10.1016/j.humimm.2012.05.007. Epub 2012 May 15.
The intercellular adhesion molecule-1 (ICAM-1) mediates interaction of activated endothelial cells with leukocytes. It plays an important role in the pathogenesis of atherosclerosis. A functionally important polymorphism of the ICAM-1 gene, K469E, has been described. We investigated whether this polymorphism influences the risk of CVD in end-stage renal disease (ESRD) patients. The groups of 1016 ESRD patients and 824 healthy individuals were genotyped by PCR and allele specific oligonucleotide technique. The T allele of the K469E polymorphism was significantly more frequent in ESRD CVD+ patients than CVD- and controls (OR 2.26, 95% CI 1.87-2.72 and 1.82, 95% CI 1.55-2.11, respectively). The TT genotype was also more frequent in CVD+ patients (OR 9.90, 95% CI 6.17-15.88 vs. CVD- subgroup). When patients were stratified according to clinical outcome of CVD, there was a tendency towards higher frequencies of the T allele and TT genotype in patients with myocardial infarction (OR for T allele 1, 57, 95% CI 1.12-2.18 vs. patients without MI). In the multivariate regression analysis the carrier status of T allele of K469E was an independent risk factor of susceptibility to CVD. Our data suggest that the ICAM-1 K469E polymorphism is associated with CVD in ESRD patients.
细胞间黏附分子-1（ICAM-1）介导活化的内皮细胞与白细胞的相互作用。它在动脉粥样硬化的发病机制中起重要作用。已经描述了 ICAM-1 基因的一种功能重要的多态性，即 K469E。我们研究了这种多态性是否会影响终末期肾病（ESRD）患者的 CVD 风险。通过 PCR 和等位基因特异性寡核苷酸技术对 1016 名 ESRD 患者和 824 名健康个体的组进行了基因分型。K469E 多态性的 T 等位基因在 ESRD CVD+患者中明显比 CVD-和对照组更为常见（OR 2.26，95%CI 1.87-2.72 和 1.82，95%CI 1.55-2.11）。TT 基因型在 CVD+患者中也更为常见（OR 9.90，95%CI 6.17-15.88 与 CVD-亚组相比）。当根据 CVD 的临床结果对患者进行分层时，心肌梗死患者 T 等位基因和 TT 基因型的频率有升高的趋势（T 等位基因的 OR 为 1.57，95%CI 1.12-2.18 与没有 MI 的患者相比）。在多元回归分析中，K469E 的 T 等位基因携带者状态是 CVD 易感性的独立危险因素。我们的数据表明，ICAM-1 K469E 多态性与 ESRD 患者的 CVD 相关。
