The Scripps Research Institute, La Jolla, CA, 92037.
Mass Spectrom Rev. 2019 Nov;38(6):445-460. doi: 10.1002/mas.21599. Epub 2019 Aug 12.
Progress in proteomics research has led to a demand for powerful analytical tools with high separation efficiency and sensitivity for confident identification and quantification of proteins, posttranslational modifications, and protein complexes expressed in cells and tissues. This demand has significantly increased interest in capillary electrophoresis-mass spectrometry (CE-MS) in the past few years. This review provides highlights of recent advances in CE-MS for proteomics research, including a short introduction to top-down mass spectrometry and native mass spectrometry (native MS), as well as a detailed overview of CE methods. Both the potential and limitations of these methods for the analysis of proteins and peptides in synthetic and biological samples and the challenges of CE methods are discussed, along with perspectives about the future direction of CE-MS. @ 2019 Wiley Periodicals, Inc. Mass Spec Rev 00:1-16, 2019.
蛋白质组学研究的进展使得人们对具有高效分离效率和高灵敏度的分析工具产生了需求，这些工具可以用于对细胞和组织中表达的蛋白质、翻译后修饰和蛋白质复合物进行可靠的鉴定和定量。在过去的几年中，毛细管电泳-质谱联用（CE-MS）受到了极大的关注。本文综述了近年来 CE-MS 在蛋白质组学研究中的最新进展，其中包括对自上而下质谱和天然质谱（native MS）的简短介绍，以及对 CE 方法的详细概述。讨论了这些方法在合成和生物样本中分析蛋白质和肽的潜力和局限性，以及 CE 方法面临的挑战，并对 CE-MS 的未来发展方向进行了展望。@ 2019 年 Wiley 期刊，Inc. 质谱评论 00：1-16，2019 年。
