Department of Bioproducts and Biosystems Engineering, University of Minnesota, 2004 Folwell Avenue, Saint Paul, MN 55108, USA.
Bioresour Technol. 2012 Jul;116:147-54. doi: 10.1016/j.biortech.2012.04.018. Epub 2012 Apr 13.
Brown rot fungi Gloeophyllum trabeum and Postia placenta were used to degrade aspen, spruce, or corn stover over 16 weeks. Decayed residues were saccharified using commercial cellulases or brown rot fungal extracts, loaded at equal but low endoglucanase titers. Saccharification was then repeated for high-yield samples using full strength commercial cellulases. Overall, brown rot pretreatments enhanced yields up to threefold when using either cellulase preparation. In the best case, aspen degraded 2 weeks by G. trabeum yielded 72% glucose-from-cellulose, a 51% yield relative to original glucan. A follow-up trial with more frequent harvests showed similar patterns and demonstrated interplay between tissue modifications and saccharification. Hemicellulose and vanillic acid (G6) or vanillin (G4) lignin residues were good predictors of saccharification potential, the latter notable given lignin's potential active role in brown rot. Results show basic relationships over a brown rot time course and lend targets for controlling an applied bioconversion process.
在 16 周的时间里，使用栓菌（Gloeophyllum trabeum）和彩绒革盖菌（Postia placenta）来降解白杨、云杉或玉米秸秆。使用商业纤维素酶或褐腐真菌提取物对腐烂的残留物进行糖化，添加的内切葡聚糖酶的效价相等但处于低端。然后，使用全强度商业纤维素酶对高产样品进行重复糖化。总体而言，当使用任何一种纤维素酶制剂时，褐腐预处理可将产率提高 2 至 3 倍。在最佳情况下，栓菌处理的白杨在 2 周内降解，可从纤维素中获得 72%的葡萄糖，相对于原始葡聚糖的产率为 51%。随后进行了更频繁收获的后续试验，结果显示出相似的模式，并证明了组织修饰和糖化之间的相互作用。半纤维素和香草酸（G6）或香草醛（G4）木质素残基是糖化潜力的良好预测因子，鉴于木质素在褐腐中的潜在积极作用，后者尤为重要。结果显示了在褐腐时间过程中的基本关系，并为控制应用生物转化过程提供了目标。
