Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, China.
Bioresour Technol. 2012 Aug;117:72-9. doi: 10.1016/j.biortech.2012.04.061. Epub 2012 Apr 26.
In this study, 454-pyrosequencing technology was employed to investigate the microbial communities in 12 municipal wastewater treatment plants (WWPTs) with different treatment processes. In total, 202,968 effective sequences of the 16S rRNA gene were generated from 16 samples that widely represented the diversity of the microbial communities. While Proteobacteria was found to be the dominant phylum in some samples, in other samples it was Bacteroidetes. The Simpson's diversity index and evenness index were lowest in samples from membrane bioreactors (MBRs), possibly due to the long sludge retention time (SRT) and low food/microorganism ratio (F/M). For one WWTP which had two disparate treatment processes operating in parallel, the structures of microbial communities in the two systems were compared. The differences found between the two indicated that the treatment process likely had effects on the structure of microbial communities.
本研究采用 454 焦磷酸测序技术，对采用不同处理工艺的 12 座城市污水处理厂（WWPT）中的微生物群落进行了研究。共从 16 个样本中生成了 202968 条 16S rRNA 基因的有效序列，这些样本广泛代表了微生物群落的多样性。虽然在一些样本中发现变形菌门是优势门，但在其他样本中却是拟杆菌门。膜生物反应器（MBR）中的样本的 Simpson 多样性指数和均匀度指数最低，这可能是由于较长的污泥停留时间（SRT）和较低的食物/微生物比（F/M）所致。对于一个同时采用两种截然不同处理工艺的 WWPT，比较了两个系统中微生物群落的结构。两个系统之间的差异表明，处理工艺可能对微生物群落的结构有影响。
