Tel Osman Yaşar, Aslantaş Ozkan, Keskin Oktay, Yilmaz Ebru Sebnem, Demir Cemil
Harran University Department of Microbiology, Faculty of Veterinary Medicine Eyyubiye Campus Eyyubiye, Sanliurfa Turkey.
Acta Vet Hung. 2012 Jun;60(2):189-97. doi: 10.1556/AVet.2012.016.
In this study, Staphylococcus aureus strains (n = 110) isolated from seven ewe flocks in Sanliurfa, Turkey were screened for antibiotic resistance and biofilmforming ability as well as for genes associated with antibiotic resistance and biofilm-forming ability. All isolates were found to be susceptible to oxacillin, gentamicin, clindamycin, cefoxitin, tetracycline, vancomycin, amoxicillin-clavulanic acid, ciprofloxacin and sulphamethoxazole-trimethoprim. The percent proportions of strains resistant to penicillin G, ampicillin and erythromycin were 27.2% (n = 30), 25.4% (n = 28) and 6.3% (n = 7), respectively. Regarding the antibiotic resistance genes, 32 (29%) isolates carried the blaZ and 8 (7.2%) the ermC gene. Other resistance genes were not detected in the isolates. All isolates showed biofilm-forming ability on Congo red agar (CRA), while 108 (98.18%) and 101 (91.81%) of them were identified as biofilm producers by the use of standard tube (ST) and microplate (MP) methods, respectively. All isolates carried the icaA and icaD genes but none of them harboured the bap gene. The results demonstrated that S. aureus isolates from gangrenous mastitis were mainly resistant to penicillins (which are susceptible to the staphylococcal beta-lactamase enzyme), and less frequently to erythromycin. Furthermore, all of the S. aureus isolates produced biofilm which was considered a potential virulence factor in the pathogenesis of staphylococcal mastitis.
在本研究中，对从土耳其桑尼乌法的七个母羊群中分离出的110株金黄色葡萄球菌菌株进行了抗生素耐药性、生物膜形成能力以及与抗生素耐药性和生物膜形成能力相关基因的筛选。所有分离株对苯唑西林、庆大霉素、克林霉素、头孢西丁、四环素、万古霉素、阿莫西林-克拉维酸、环丙沙星和磺胺甲恶唑-甲氧苄啶敏感。对青霉素G、氨苄西林和红霉素耐药的菌株比例分别为27.2%（n = 30）、25.4%（n = 28）和6.3%（n = 7）。关于抗生素耐药基因，32株（29%）分离株携带blaZ基因，8株（7.2%）携带ermC基因。在分离株中未检测到其他耐药基因。所有分离株在刚果红琼脂（CRA）上均表现出生物膜形成能力，而分别使用标准试管（ST）法和微孔板（MP）法时，其中108株（98.18%）和101株（91.81%）被鉴定为生物膜产生菌。所有分离株均携带icaA和icaD基因，但均未携带bap基因。结果表明，来自坏疽性乳腺炎的金黄色葡萄球菌分离株主要对青霉素（易被葡萄球菌β-内酰胺酶酶解）耐药，对红霉素耐药的频率较低。此外，所有金黄色葡萄球菌分离株均产生生物膜，这被认为是葡萄球菌乳腺炎发病机制中的一种潜在毒力因子。
